Evaluation of the effects of antibiotics on cytotoxicity of EGFP and DsRed2 fluorescent proteins used for stable cell labeling

• Authors: Karolina Zakrzewska , Anna Samluk , Krzysztof Pluta , Dorota Pijanowska
• Languages of publication: EN

Abstracts

EN

The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of the EGFP and DsRed2 markers used for whole-cell labeling. Results showed that doxycycline negatively affected albumin synthesis in DsRed2-expressing hepatoma cells, and that both hepatoma cells and human skin fibroblasts, labeled with this protein, were characterized by the lowered growth rates. Thus, the cytotoxic effect of fluorescent markers depends on both protein used for cell labeling and on growth conditions that may cause cell stress.

Keywords

EN

stable fluorescent labeling; whole-cell labeling; fluorescent protein cytotoxicity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 809-813

Dates

published

2014

received

2014-06-17

revised

2014-10-09

accepted

2014-10-17

(unknown)

2014-11-07

Contributors

author

Karolina Zakrzewska

• Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland

author

Anna Samluk

• Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland

author

Krzysztof Pluta

• Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland

author

Dorota Pijanowska

• Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland

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