Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis

• Authors: Małgorzata Czernicka , Jarosław Pławiak , Piotr Muras
• Languages of publication: EN

Abstracts

EN

Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.

Keywords

EN

Betula nana; Betula utilis 'Doorenbos'; interspecific hybrids; ploidy analysis; RAPD-PCR

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 2
• Pages: 195-199

Dates

published

2014

received

2013-10-15

revised

2014-04-06

accepted

2014-04-17

(unknown)

2014-06-06

Contributors

author

Małgorzata Czernicka

• Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, Kraków, Poland

author

Jarosław Pławiak

• Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, Kraków, Poland

author

Piotr Muras

• Department of Dendrology and Landscape Architecture, University of Agriculture in Krakow, Kraków, Poland

References

• Anamthawat-Jónsson K (2003) Preparation of chromosomes from plant leaf meristems for karyotype analysis and in situ hybridization. Methods in Cell Science 25: 91-95.
• Atkinson MD (1992) Betula pendula Roth (B. verrucosa Ehrh.) and B. pubescens Ehrh. J Ecol 80: 837-870.
• Darnell JE, Baltimore D, Lodish IIF (1990) Molecular and cellular biology. Scientific American Books, New York.
• Dąbrowska G, Dzialuk A, Burnicka O, Ejankowski W, Gugnacka-Fiedor W, Goc A (2006) Genetic diversity of postglacial relict shrub Betula nana revealed by RAPD analysis. Dendrobiology 55: 19-23.
• De Groot WJ, Thomas PA, Wein RW (1997) Betula nana L. and Betula glandulosa Michx. Biological Flora of the British Isles. Journal of Ecology 85: 241-264.
• Eaker TA, Ranney TG, Viloria ZJ, Mowrey JA (2004) Variation in ploidy level among birch taxa. Plant Breeding & Evaluation Section. SNA Research conference 49: 548-551.
• Eifler I (1960) Untersuchungen zur individuellen Bedingtheit des Kreuzungserfolges zwischen Betula pendula and Betula pubescens. Silvae Genet 9: 159-165.
• Ejankowski W, Kunz M (2006) Reconstruction of vegetation Dynamics in 'Linje' peat - bog (N Poland) using remote sensing metod. Biodiv Res Conserv 1-2: 111-113.
• Felsenstein J (2005) PHYLIP (Phylogeny Inference Package) version 3.65. Distributed by the author. Department of Genetics, University of Washington, Seattle.
• Furlow J (1990) The genera of Betulaceae in the southeastern United States. Journal of the Arnold Arboretum 71: 1-67.
• Hagman M (1971) On self and cross incompatibility shown by Betula verrucosa Ehrh. and B. pubescens Ehrh. Commun Inst For Fenn 73: 1.
• Järvinen P, Palmé A, Morales LO, Lännenpää M, Keinänen M, Sopanen T, Lascoux M (2004) Phylogenetic relationships of Betula species (Betulaceae) based on nuclear ADH and chloroplast matK sequences. Am J Bot 91: 1834-1845.
• Jadwiszczak KA, Drzymulska D, Banaszek A, Jadwiszczak P (2012) Population history, genetic variation and conservation status of the endangered birch species Betula nana L. in Poland. Silva Fennica 46: 465-477.
• Johnsson H (1945) Interspecific hybridisation within the genus Betula. Hereditas 31: 163-176.
• Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci 70: 3321-3323.
• Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6: 288-295.
• Roy A, Frascaria N, MacKay J, Bousquet J (1992) Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis. Theor Appl Genet 85: 173-180.
• Schenk M, Thienpont CN, Koopman W, Gilissen L, Smulders M (2008) Phylogenetic relationships in Betula (Betulaceae) based on AFLP markers. Tree Genet Genomes 4: 911-924.
• Thorsson AT, Palsson S, Sigurgeirsson A, Anamthawat - Jonsson K (2007) Morphological variation among Betula nana (diploid), B. pubescens (tetraploid) and their triploid hybrids in Iceland. Ann Bot 99: 1183-1193.
• Tsuda Y, Goto S, Ide Y (2004) RAPD analysis of genetic variation within and among four natural populations of Betula maximowicziana. Silvae Genetica 53: 234-239.
• Tyagi BR, Ahmed T, Bahl JR (1992) Cytology, genetics and breeding of commercially important Mentha species. Curr Res Med Arom Plants 14: 51-56.
• Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
• Zeng J, Zou Y, Bai J, Zheng H (2003) RAPD analysis of genetic variation in natural populations of Betula alnoides from Guangxi, China. Euphytica 134: 33-41.