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2013 | 60 | 4 | 861-864

Article title

Temperature effect on growth, and selected parameters of Phaeodactylum tricornutum in batch cultures

Content

Title variants

Languages of publication

EN

Abstracts

EN
The effect of optimal and stress temperatures on the growth kinetics of the Phaeodactylum tricornutum CCAP/1055/1 strain (a model diatom with a known genome sequence) in batch cultures was examined. The analysis of the obtained results showed two phases of culture growth. There were significant positive correlations between OD increase of chlorophyll a chlorophyll c and protein concentration at different temperatures. The Fv/Fm parameter achieved a maximum level on the 6th or 7th day and then decreased to the values registered on the first day of observation. Genetic material undergoes gradual degradation 10 days after inoculation. The size of the cells was invariable.

Year

Volume

60

Issue

4

Pages

861-864

Physical description

Dates

published
2013
received
2013-10-31
revised
2013-12-09
accepted
2013-12-17

Contributors

author
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

References

  • Fawley MW (1984) Effects of light intensity and temperature interaction on growth characteristic of Phaeodactylum tricornutum (Bacillariophyceae). J Phycol 20: 67-72.
  • Goossens K (2011) Engineering of lipid metabolism in the diatom Phaeodactylum tricornutum Master's dissertation submitted to obtain the degree of Master of Biochemistry and Biotechnology Major Structural Biotechnology and Biochemistry, Academic year 2011-2012.
  • Jeffrey SW, Humphrey GF (1975) New spectrophotometric equation for determining chlorophyll a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanz 167: 191-194.
  • Kudo I, Miyamoto M, Noiri Y, Maita Y (2000) Combined effects of temperature and iron on the growth and physiology of the marine diatom Phaeodactylum tricornutum (Bacillariophyceae). J Physiol 36: 1096-1102.
  • Liang Y, Beardall J, Heraud P (2006) Effect of UV radiation on growth, chlorophyll fluorescence and fatty acid composition of Phaeodactylum tricornutum and Chaetoceros muelleri (Bacillariophyceae). Phycologia 45: 605-615.
  • Lichtenthaler HK, Buschmann C, Knapp M (2005) How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer. Photosynthetica 43: 379-393.
  • Lowry OH, Rosebrough NJ, Farr A., Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-227.
  • Martino AD, Bartual A, Willis A, Meichenin A, Villazán B, Maheswari U, Bowler C (2011) Physiological and molecular evidence that environmental changes elicit morphological interconversion in model diatom Phaeodactylum tricornutum. Protist 162: 462-481.
  • Maxwell K, Johnson GN (2000) Chlorophyll fluorescence - a practical guide. J Exp Bot 51: 659-668.
  • Yongmanitchai W, Ward P (1991) Growth of Omega-3 fatty acid production by Phaeodactylum tricornutum under different culture condition. Appl Environ Micriobiol 57: 419-425.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv60p861kz
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