Influence of process parameters on properties of Nanostructured Lipid Carriers (NLC) formulation

• Authors: Elwira Lasoń , Elżbieta Sikora , Jan Ogonowski
• Languages of publication: EN

Abstracts

EN

Nanostructured lipid carriers (NLC) are stable colloidal formulations with notable advantages for drug delivery systems. Thanks to their physicochemical stability, biocompatibility, biodegradability and controlled drug release, they have received increasing attention for the last several years. The aim of the study was to prepare and characterize nanostructured lipid carriers (NLC). Both, the effect of the process parameters and the effect of the preemulsion composition on the NLC properties were investigated. In the work, different type of surfactants (i.e. decyl glucoside, Poloxamer188, Tween 80, sodium cholate) and their combinations were used to stabilize NLC dispersions. Moreover, several kinds of solid lipids (modified beeswax, gliceryl behenate, cetyl palmitate and berry wax) and liquid lipids (caprilic/capric triglyceride and decyl oleate) were applied. An ultrasonication method using a probe type sonicator was used to obtain NLC, and the time and energy of the process were modified throuhout. The physicochemical properties of the formulations, such as particle size, size distribution, polidispersity index were studied using the dynamic light scattering (DLS) method. The electrophoretic mobility of obtained particles was also measured, using the Zetasizer Nano ZS Malvern Instrument based on the Laser Doppler Velocimetry (LDV) technique. Knowing the value of electrophoretic mobility of particles for given conditions, the zeta potential was determined. The obtained results showed that the process parameters and the composition of the preemulsion had significant impact on the nanoparticles structure. The optimal formulations size ranged between 60 and 80 nm, and the value of their zeta potential was up to -30mV. The stability of these systems was further confirmed by macroscopic observation.

Keywords

EN

nanostructured lipid carriers; NLC; liquid lipids; solid lipids; ultrasonication

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 773-777

Dates

published

2013

received

2013-10-14

revised

2013-12-04

accepted

2013-12-04

Contributors

author

Elwira Lasoń

• Institute of Organic Chemistry and Technology, Cracow University of Technology, Kraków, Poland

author

Elżbieta Sikora

• Institute of Organic Chemistry and Technology, Cracow University of Technology, Kraków, Poland

author

Jan Ogonowski

• Institute of Organic Chemistry and Technology, Cracow University of Technology, Kraków, Poland

References

• Almeida AJ, Souto E (2007) Solid lipid nanoparticles as a drug delivery system for peptides and proteins. Adv Drug Deliv Rev 59: 478-490.
• Chen CC, Tsai TH, Huang ZR, Fang JY (2010) Effects of lipophilic emulsifier on the oral administration of lovastin from nanostructured lipid carriers: Physicochemical characterization and pharmacokinetics. Eur J Pharm Biopharm 74: 474-482.
• Das S, Chaudhury A (2011) Recent advances in lipid nanoparticle formulations with solid matrix for oral drug delivery. AAPS PharmSciTech 12: 62-76.
• Han F, Li S, Yin R, Liu HZ, Xu L (2008) Effect of surfactant on the formation and characterization of a new type of colloidal drug delivery system: nanostructured lipid carriers. Colloids Surf A Physicochem Eng Asp 315: 210-216.
• Hu FQ, Jiang SP, Du YZ, Yuan H, Ye Y, Zeng S (2006) Preparation and characteristics of monostearin nanostructured lipid carriers. Int J Pharm 314: 83-89.
• Jain NK, Ram A (2011) Development and characterization of nanostructured lipid carriers of oral hypoglycemic agent: selection of surfactants. Int J Pharm Sci Rev Res 7: 125-130.
• Kovacevic A, Savic S, Vuleta G, Müller RH, Keck CM (2011) Polyhydroxy surfactants for the formulation of lipid nanoparticles (SLN and NLC): effect on size, physical stability and particle matrix structure. Int J Pharm 406: 163-172.
• Müller RH, Mehnert W, Lucks JS, Schwarz C, zur Muhlen A, Weyhers H, Freitas C, Ruhl D (1995) Solid lipid nanoparticles (SLN): an alternative colloidal carrier system for controlled drug delivery. Eur J Pharm Biopharm 41: 62-69.
• Müller RH, Petersen RD, Hommoss A, Pardeike J (2007) Nanostructured lipid carriers (NLC) in cosmetic dermal products. Adv Drug Deliv Rev 59: 522-530.
• Müller RH, Radtke M, Wissing SA (2002a) Nanostructured lipid matrices for improved microencapsulation of drugs. Int J Pharm 242: 121-128.
• Pardeike J, Hommos A, Müller RH (2009) Lipid nanoparticles (SLN, NLC) in cosmetic and pharmaceutical dermal products. Int J Pharm 366: 170-184.
• Radtke M, Souto EB, Müller RH (2005) Nanostructured lipid carriers: a novel generation of solid lipid drug carriers. Pharm Technol Eur 17: 45-50.
• Saraf S, Gosh A, Kaur CD, Saraf S (2011a) Novel modified nanosystem based lymphatic targeting. Res J Nanosci Nanotechnol 1: 60-74.
• Saraf S, Rathi R, Kaur CD, Saraf S (2011b) Colloidosomes an Advanced Vesicular System in Drug Delivery. Asian J Sci Res 4: 1-15.
• Sawant KK, Dodiya SS (2008) Recent advances and patents on solid lipid nanoparticles. Recent Pat Drug Deliv Formulat 2: 120-135.
• Schafer-Korting M, Mehnert W, Korting HC (2007) Lipid nanoparticles for improved topical application of drugs for skin diseases. Adv Drug Deliv Rev 59: 427-443.
• Schwarz JC, Weixelbaum A, Pagitsch E, Löw M, Resch GP, Valenta C (2012) Nanocarriers for dermal drug delivery: Influence of preparation method, carrier type and rheological properties. Int J Pharm 437: 83-88.
• Siekmann B, Westesen K (1994) Melt homogenized solid lipid nanoparticles stabilized by the nonionic surfactant tylaxopol. Preparation and particle size determination. Pharm Pharmacol Lett 3: 194-197.
• Westesen K, Bunjes H, Koch MHJ (1997) Physicochemical characterization of lipid nanoparticles and evaluation of their drug loading capacity and sustained release potential. J Control Release 48: 223-236.
• Zheng M, Falkeborg M, Zheng Y, Yang T, Xu X (2013) Formulation and characterization of nanostructured lipid carriers containing a mixed lipids core. Colloids Surf A Physicochem Eng Asp 430: 76-84.