Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers

• Authors: Adam Junka , Stanisław Deja , Danuta Smutnicka , Patrycja Szymczyk , Grzegorz Ziółkowski , Marzenna Bartoszewicz , Piotr Młynarz
• Languages of publication: EN

Abstracts

EN

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.

Keywords

EN

Staphylococcus aureus; biofilm; NMR; metabolomics; glycine-betaine

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 701-706

Dates

published

2013

received

2013-10-31

revised

2013-11-25

accepted

2013-12-12

Contributors

author

Adam Junka

• Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland

author

Stanisław Deja

• Faculty of Chemistry, Opole University, Opole, Poland

author

Danuta Smutnicka

• Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland

author

Patrycja Szymczyk

• Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland

author

Grzegorz Ziółkowski

• Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland

author

Marzenna Bartoszewicz

• Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland

author

Piotr Młynarz

• Faculty of Chemistry, Opole University, Opole, Poland

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