Evaluation of the activity of thermostable DNA polymerases in the presence of heme, as a key inhibitor in the real time PCR method in diagnostics of sepsis

• Authors: Tomasz Gosiewski , Monika Brzychczy-Włoch , Agata Pietrzyk , Agnieszka Sroka , Małgorzata Bulanda
• Languages of publication: EN

Abstracts

EN

The study aim was evaluation of the usefulness of several thermostable DNA polymerases in real time PCR conducted in the presence of the heme. Our study had the advantage of testing several different polymerases, one of which proved to be the least sensitive to heme activity. We also found that there is no need of supplementing the reaction mixture with protective substances like BSA. Selection of the appropriate polymerase can increase the efficiency of the PCR reaction which is very important for diagnosis of sepsis and for other analyses performed on DNA template isolated from the blood.

Keywords

EN

polymerase inhibitor; heme; real time PCR; sepsis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 603-606

Dates

published

2013

received

2013-06-06

revised

2013-10-08

accepted

2013-11-12

(unknown)

2013-12-17

Contributors

author

Tomasz Gosiewski

• Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Monika Brzychczy-Włoch

• Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Agata Pietrzyk

• Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Agnieszka Sroka

• Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Małgorzata Bulanda

• Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

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