Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 59 | 4 | 631-638

Article title

The relationship between morphology and disaccharidase activity in ischemia- reperfusion injured intestine

Content

Title variants

Languages of publication

EN

Abstracts

EN
Background: Questions regarding functional markers characterizing injured intestines remain unanswered. Brush border disaccharidases are crucial for the functioning of the intestines. Aims: The study was designed to assess changes in disaccharidase activity (DA) following intestinal injury and to compare them with morphological changes. Methods: Wistar rats, randomly divided into six experimental groups (each n = 6), were subjected to different ischemic/reperfusion injury. One-hour mesenteric ischemia followed by reperfusion for 0, 1, 2, 4, 12 or 24 hours was induced. As a control group sham-operated animals were used (n = 6). Intestine morphology was evaluated using histopathological injury index (HII) and goblet cell (GC) detection. DA (sucrase and maltase) was studied in mucosal scrape or in entire intestinal wall samples. Results: Moderate morphological damage (HII, GC) after mesenteric ischemia was detected. Deepening of the injury was found during reperfusion with a maximum after two hours. Improved morphology with longer reperfusion confirmed reversible damage with almost normal mucosal structure after 24 hours of reperfusion. Similar pattern was observed when DA was measured. The lowest activity was detected after 2 hours of reperfusion followed by increasing activity in the subsequent reperfusion periods. Physiological values after 24 hours of reperfusion were seen only in samples of entire intestinal wall. Conclusions: Significant changes in intestinal DA were observed after intestinal ischemia/reperfusion injury. A similar pattern was seen for morphological characteristics. Although based on microscopic survey the intestine seems to be fairly regenerated, some functional limitation is expected to persist.

Year

Volume

59

Issue

4

Pages

631-638

Physical description

Dates

published
2012
received
2012-06-04
revised
2012-10-29
accepted
2012-11-26
(unknown)
2012-11-30

Contributors

author
  • 2nd Department of Obstetrics and Gynecology, P. J. Šafárik University and L. Pasteur University Hospital, Košice, Slovak Republic
  • Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia
author
  • Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia
  • Department of Chemistry, Biochemistry, Medical Biochemistry and LABMED, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia
  • Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia
  • Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia

References

  • Aramayo LA (1983) Disaccharidase activities in jejunal fluid. Arch Dis Childhood 58: 686-691.
  • Arcuni J, Wang L, Franson R, Sonnino RE (2000) Modification of disaccharidase activity measurement in rat bowel. J Invest Surg 13: 29-33.
  • Bradford M (1976) A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
  • Cunningham CC, Spach PI (1987) The effect of chronic ethanol consumption on the lipids in liver mitochondria. Ann N Y Acad Sci USA 492: 181-192.
  • Cuong DT, Johanna C, Cory JX (2011) Dietary zinc and metallothionein on small intestinal disaccharideses activity in m ice. World J Gastroenterol 17: 354-360.
  • Dahlqvist A (1968) Assay of intestinal disaccharidases. Anal Biochem 22: 99-107.
  • Duff M, Ettarh RR (2002) Crypt cell production rate in the small intestine of the zinc-supplemented mouse. Cells Tissues Organs 172: 21-28.
  • Fernandes MI, Galvao LC, Bortolozzi MF, Oliveira WP, Zucoloto S, Bianchi ML (1997) Disaccharidase levels in normal epithelium of the small intestine of rats with iron deficiency anemia. Braz J Med Biol Res 30: 849-854.
  • Gebhard RL, Karouani R, Prigge WF, McClain CJ (1983) The effect of severe zinc deficiency on activity of intestinal disaccharidases and 3-hydroxy-3-methylglutaryl coenzyme A reductase in the rat. J Nutr 113: 855-859.
  • Grootjans J, Lenaerts K, Derikx JP, Matthijsen RA, de Bruine AP, van Bijnen AA, van Dam RM, Dejong CH, Buurman WA (2010) Human intestinal ischemia-reperfusion-induced inflammation characterized: experiences from a new translational model. Am J Pathol 176: 2283-2291.
  • Gupta SK, Chong SK, Fitzgerald JF (1999) Disaccharidase activities in children: normal values and comparison based on symptoms and histologic changes. J Pediatr Gastroenterol Nutr 28: 246-251.
  • Hedemann MS, Jensen BB, Poulsen HD (2006) Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs. J Anim Sci 84: 3310-3320.
  • Huang CC, Chen JR, Liu CC, Chen KT, Shieh MJ, Yang SC (2005) Effects of long-term ethanol consumption on jejunal lipase and disaccharidase activities in male and female rats. World J Gastroenterol 11: 2603-2608.
  • Ikeda H, Yang CL, Tong J, Nishimaki H, Masuda K, Takeo T, Kasai K, Itoh G (2002) Rat small intestinal goblet cell kinetics in the process of restitution of surface epithelium subjected to ischemia-reperfusion injury. Dig Dis Sci 47: 590-601.
  • Kaufman SS, Wisecarver JL, Ruby EI, Fox IJ, Sudan DL, Pillen TJ, Dhawan A, Horslen SP, Vanderhoof JA, Radio SJ, Markin RS, Langnas AN (1998) Correlation of mucosal disaccharidase activities with histology in evaluation of rejection following intestinal transplantation. Pediatr Transplant 2: 134-138.
  • Kim YS, Ho SB (2010) Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroenterol Rep 12: 319-330.
  • Kjellev S (2009) The trefoil factor family - small peptides with multiple functionalites. Cell Mol Life Sci 66: 1350-1369.
  • Kuusanmäki P, Lauronen J, Paavonen T, Pakarinen M, Savilahti E, Häyry P, Halttunen J (1996) Brush border enzymes in evaluation of acute small bowel rejection. Scand J Immunol 43: 566-573.
  • Kuusanmäki P, Lauronen J, Paavonen T, Pakarinen M, Yilmaz S, Häyry P, Halttunen J (1997) How to diagnose chronic rejection. A study in porcine intestinal allografts. Scand J Immunol 46: 514-519.
  • Li Y, Zhu L, Li J (2006) Two-step procedure of whole orthotopic intestinal transplantation in rats: considerations of techniques and graft functional adaptation. Microsurgery 26: 399-403.
  • Lin CH, Correia L, Tolia K, Gesell MS, Tolia V, Lee PC, Luk GD (1998) Early weaning induces jejunal ornithine decarboxylase and cell proliferation in neonatal rats. J Nutr 128: 1636-1642.
  • Nano JL, Cefai D, Rampal P (1990) Effects of ethanol on an intestinal epithelial cell line. Alcohol Clin Exp Res 14: 32-37.
  • Oku T, Tanabe K, Ogawa S, Sadamori N, Nakamura S (2011) Similarity of hydrolyzing activity of human and rat small intestinal disaccharidases. Clin Exp Gastroenterol 4: 155-161.
  • Oltean M, Pullerits R, Zhu C, Blomgren K, Hallberg EC, Olausson M (2007) Donor pretreatment with FK 506 reduces reperfusion injury and accelerates intestinal graft recovery in rats. Surgery 141: 667-677.
  • Payne RL, Bidner TD, Fakler TM, Southern LL (2006) Growth and intestinal morphology of pigs from sows fed two zinc sources during gestation and lactation. J Anim Sci 84: 2141-2149.
  • Perreault C, Jutras J, Roy DC, Filep JG, Brochu S (1996) Identification of an immunodominant mouse minor histocompatibility antigen (MiHA). T cell response to a single dominant MiHA causes graft-versus-host disease. J Clin Invest 98: 622-628.
  • Pluske JR, Thompson MJ, Atwood CS, Bird PH, Williams IH, Hartmann PE (1996) Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows' whole milk after weaning. Br J Nutr 76: 409-422.
  • Porter CW, Dworacyzk D, Gurtoo HL (1982) Biochemical localization of aryl hydrocarbon hydroxylase in the intestinal epithelium of the rat. Cancer Res 42: 1283-1285.
  • Prasad KK, Thapa BR, Nain CK, Sharma AK, Singh K (2008) Brush border enzyme activities in relation to histological lesion in pediatric celiac disease. J Gastroenterol Hepatol 23: e348-e352.
  • Quaedackers JS, Beuk RJ, Bennet L, Charlton A, oude Egbrink MG, Gunn AJ, Heineman E (2000) An evaluation of methods for grading histologic injury following ischemia/reperfusion of the small bowel. Transplant Proc 32: 1307-1310.
  • Raul F (1988) Age related increase of brush border enzyme activities along the small intestine. Gut 29: 1557-1563.
  • Rodriguez-Castilla J, Lopez-Nuevo M, Delgado MJ, Murillo ML, Carreras O (1996) Changes in the ileal disaccharidases activities in the rats after long-term ethanol feeding. Alcohol Alcohol 31: 69-74.
  • Sabat P, Veloso C (2003) Ontogenic development of intestinal disaccharidases in the precocial rodent Octodon degus (Octodontidae). Comp Biochem Physiol A Mol Integr Physiol 134: 393-397.
  • Stallion A, Kou TD, Miller KA, Dahms BB, Dudgeon DL, Levine AD (2002) IL-10 is not protective in intestinal ischemia reperfusion injury. J Surg Res 105: 145-152.
  • Su B (1998) Study progress on apoptosis and ischemia-reperfusion injury. Foreign Med Surg 25: 325-327.
  • Taguchi T, Yamada T, Suita S, Ohta M (1995) The significance of cytological examination on reperfusion in rat small intestinal transplantation. Virchows Arch 427: 71-76.
  • Taupin D, Podolsky DK (2003) Trefoil factors: initiators of mucosal healing. Nat Rev Mol Cell Biol 4: 721-732.
  • Teitelbaum DH, Wise WE, Sonnino RE, Dunaway DJ, Powers P, McClung HJ, Harmel RP Jr (1989) Monitoring of intestinal transplant rejection. Am J Surg 157: 318-322.
  • Tori AJ, Carroll AE, Gupta SK (2007) Disaccharidase activity in infants and comparison based on symptoms and histological changes. J Pediatr Gastroenterol Nutr 45: 194-198.
  • Toth Š, Pomfy M, Wohlfahrt P, Pingorová S, Kišš J, Baláž P, Rokošný S, Beňačka R, Veselá J (2007) Detection of early stages of apoptosis in experimental intestinal ischemia-reperfusion injury. Biologia 62: 491-497.
  • Tóth S, Jonecová Z, Varga J, Staško P, Kovavalčinová B, Maretta M, Veselá J (2012) Mesenteric ischemia-reperfusion injury: Specific impact on different cell populations within the jejunal wall in rats. Acta Histochemica 114: 276-284
  • Tran CD, Cool J, Xian CJ (2011) Dietary zinc and metallothionein on small intestinal disaccharidases activity in mice. World J Gastroenterol 17: 354-360.
  • Varga J, Stasko P, Tóth S, Pristásová Z, Bujdos M, Pomfy M (2009) Development of jejunal graft damage during intestinal transplantation. Ann Transplant 14: 62-69.
  • Varga J, Stasko P, Tóth S, Pristášová Z, Jonecová Z, Veselá J, Pomfy M (2010) Morphological and apoptotic changes in the intestinal mucosa and lung parenchyma after ischaemic/reperfusion injury of the jejunum. Acta Vet Hung 58: 243-256.
  • Varga J, Tóth S, Staško P, Bujdoš M, Veselá J, Jonecová Z, Pomfy M (2011) Different ischemic preconditioning regimens affecting preservation injury of intestines. Eur Surg Res 46: 207-213.
  • Vieira MR, Galvão LC, Fernandes MI (2000) Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia. Braz J Med Biol Res 33: 539-544.
  • Yeh KY, Yeh M, Glass J, Granger DN (2000) Rapid activation of NF-kappaB and AP-1 and target gene expression in postischemic rat intestine. Gastroenterology 118: 525-534.
  • Yildiz Y, Kose H, Cecen S, Ergin K, Demir EM, Serter M (2010) Protective effects of leflunomide on intestinal ischemia-reperfusion injury: leflunomide against intestinal ischemia-reperfusion. Dig Dis Sci 55: 245-252.
  • Yildiz Y, Serter M, Ek RO, Ergin K, Cecen S, Demir EM, Yenisey C (2009) Protective effects of caffeic acid phenethyl ester on intestinal ischemia-reperfusion injury. Dig Dis Sci 54: 738-744.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv59p631kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.