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2011 | 58 | 4 | 529-534

Article title

Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours

Content

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Languages of publication

EN

Abstracts

EN
In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human.

Year

Volume

58

Issue

4

Pages

529-534

Physical description

Dates

published
2011
received
2011-03-21
revised
2011-09-14
accepted
2011-10-13
(unknown)
2011-11-08

Contributors

  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy
  • Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
author
  • Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland
author
  • Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland
author
  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy
  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy
  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy
  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy
author
  • Dipartimento di Biologia e Biotecnologie 'Charles Darwin' and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Università di Roma, Roma, Italy

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv58p529kz
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