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2011 | 58 | 3 | 349-353

Article title

Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants

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Abstracts

EN
Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.

Year

Volume

58

Issue

3

Pages

349-353

Physical description

Dates

published
2011
received
2011-01-20
revised
2011-07-01
accepted
2011-08-31
(unknown)
2011-09-13

Contributors

  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
author
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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Document Type

Publication order reference

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YADDA identifier

bwmeta1.element.bwnjournal-article-abpv58p349kz
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