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2011 | 58 | 3 | 297-302

Article title

The effects of velvet antler polypeptides on the phenotype and related biological indicators of osteoarthritic rabbit chondrocytes

Content

Title variants

Languages of publication

EN

Abstracts

EN
Objective: To study the effects of velvet antler polypeptides (VAPs) on osteoarthritic chondrocytes (OCs) in rabbits. Methods: An osteoarthritic rabbit model was established according to Hulth's method. OCs were isolated and cultured for observation of the cell cycle. Cell proliferation was detected by MTT assay and the cell cycle was monitored by flow cytometry. The phenotype was determined by toluidine blue staining as well as immunohistochemical staining for collagen type II. The expression of MMP-1, MMP-3, MMP-13, TIMP-1, and collagen I and X mRNA by chondrocytes was assayed by RT-PCR. Results: The VAPs had no obvious proliferative effect on OCs and did not affect the cell cycle. However, they significantly reduced the proportion of early apoptotic cells in a dose-dependent manner. Further, VAPs inhibited the expression of collagen I and X mRNA and induced abnormal expression of MMP-1 and MMP-13 mRNA. VAPs had no significant effect on MMP-3 and TIMP-1 mRNA levels. The toluidine blue and collagen type II immunohistochemical staining intensities of VAP-treated chondrocytes were positively correlated with the concentration of VAPs used. Conclusion: VAPs had no significant effect on OC proliferation and the cell cycle, but did increase the glycosaminoglycan (GAG) and collagen type II expression levels in the extracellular matrix, and down-regulated collagen I and X mRNA expression. Treatment of cartilage cells with VAPs maintained their normal phenotype, inhibited matrix metalloproteinases (MMPs) secretion, kept the balance of cartilage matrix metabolism, and sustained an external environment where the cartilage cells could survive. Moreover, VAPs reduced the proportion of early apoptotic cells, suggesting that they may block the apoptotic pathway in OCs.

Year

Volume

58

Issue

3

Pages

297-302

Physical description

Dates

published
2011
received
2010-11-11
revised
2011-05-12
accepted
2011-07-03
(unknown)
2011-07-11

Contributors

  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Clinical Laboratory of Tumor Hospital of Jilin Province, Jilin, P.R.China
author
  • Department of Pharmacology, Henan University of Science and Technology, Henan, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China

References

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  • Duan Lx (2007) Studies on the chemical structrue of velvet antler polypeptides (cervus Nippon temminck) and its anti-hepatic fibrosis. D, Jilin University.
  • Gauci SJ, Golub SB, Tutolo L, Little CB, Sims NA, Lee ER, Mackie EJ, Fosang AJ (2008) Modulating chondrocyte hypertrophy in growth plate and osteoarthritic cartilage. J Musculoskelet Neuronal Interact 8: 308-310.
  • Guo Y, Zhou Q, Liu P, Wang Y, Fang J, Wang B (1998) The research of pilose antler polypeptides promoting osteoblast precursor cells and chondrocytes proliferation. J Chinese J Biochem Pharmaceutics 19: 74-75.
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  • Liu X, Li X, Zhou J (2005) Experimental study on replicating knee osteoarthritis by modified Hulth's modeling method. J Chinese J Int Trad West Med 25: 1104-1108.
  • Pelletier JP (1990) Imbalance between the mehanisms of activation and inhibition of metallo-proteases in the early lesions of experimental osteoarthristis. Arthritis Rheum 33: 364-367.
  • Qu M, Kang Y (2000) Study on biological characteristics of hypertrophic chondrocytes of damaged articular cartilage.J Chinese J Sports Med 19: 76-81.
  • Von Der Mark K, Kirsch T, Nerlich A, Kuss A, Weseloh G, Glückert K, Stöss H (1992) Type X collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy. Arthritis Rheum 35: 806-811.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv58p297kz
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