Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology

• Authors: Karol Jelonek , Anna Walaszczyk , Dorota Gabryś , Monika Pietrowska , Chryso Kanthou , Piotr Widłak
• Languages of publication: EN

Abstracts

EN

Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects of ionizing radiation are available. Here we describe the isolation of mouse primary cardiac endothelial cells, a possible target for cardiotoxic effects of radiation. Cells isolated from hearts of juvenile mice were cultured and irradiated in vitro. In addition, cells isolated from hearts of locally irradiated adult animals (up to 6 days after irradiation) were tested. A dose-dependent formation of histone γH2A.X foci was observed after in vitro irradiation of cultured cells. However, such cells were resistant to radiation-induced apoptosis. Increased levels of actin stress fibres were observed in the cytoplasm of cardiac endothelial cells irradiated in vitro or isolated from irradiated animals. A high dose of 16 Gy did not increase permeability to Dextran in monolayers formed by endothelial cells. Up-regulated expression of Vcam1, Sele and Hsp70i genes was detected after irradiation in vitro and in cells isolated few days after irradiation in vivo. The increased level of actin stress fibres and enhanced expression of stress-response genes in irradiated endothelial cells are potentially involved in cardiotoxic effects of ionizing radiation.

Keywords

EN

cardiac endothelial cells; radiation; cardiotoxicity; primary cell culture; stress response

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2011
• Volume: 58
• Issue: 3
• Pages: 397-404

Dates

published

2011

received

2011-04-02

revised

2011-07-01

accepted

2011-08-01

(unknown)

2011-08-31

Contributors

author

Karol Jelonek

• Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

author

Anna Walaszczyk

• Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

author

Dorota Gabryś

• Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

author

Monika Pietrowska

• Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

author

Chryso Kanthou

• University of Sheffield, Sheffield, United Kingdom

author

Piotr Widłak

• Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland

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