Effects of 1-methylnicotinamide and its metabolite N-methyl-2-pyridone-5-carboxamide on streptozotocin-induced toxicity in murine insulinoma MIN6 cell line

• Authors: Tomasz Przygodzki , Ewa Slominska , Ewa Polakowska , Wojciech Mlynarski , Cezary Watala
• Languages of publication: EN

Abstracts

EN

1-methylnicotinamide (MNA) is a primary metabolite of nicotinamide. In recent years several activities of MNA have been described, such as anti-inflammatory activity in skin diseases, induction of prostacyclin synthesis via COX-2, aortal endothelium protection in diabetes and hypertriglyceridaemia and increased survival rate of diabetic rats. 1-methylnicotinamide was also suggested to protect pancreatic cells from streptozotocin in vivo. Streptozotocin toxicity is known to be mediated by poly-ADP-ribose polymerase. Nicotinamide and its derivatives have been shown to ameliorate poly-ADP-ribose polymerase-dependent nucleotide pool reduction. We aimed to verify if 1-methylnicotinamide and its metabolite, N-methyl-2-pyridone-5-carboxamide, can protect insulinoma cells from streptozotocin-induced toxicity. We found that N-methyl-2-pyridone-5-carboxamide, but not 1-methylnicotinamide, restores the pool of ATP and NAD+ in streptozotocin-treated cells, but neither compound improved the cell viability. We conclude that inhibition of poly-ADP-ribose polymerase-dependent nucleotide pool reduction may not be sufficient to protect cells from streptozotocin toxicity.

Keywords

EN

nucleotide pool; streptozotocine; 1-methylnicotinamide; N-methyl-2-pyridone-5-carboxamide

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2011
• Volume: 58
• Issue: 1
• Pages: 75-77

Dates

published

2011

received

2010-08-10

revised

2011-01-19

accepted

2011-01-24

(unknown)

2011-03-14

Contributors

author

Tomasz Przygodzki

• Department of Haemostasis and Haemostatic Disorders, Chair of Laboratory Diagnostics, Medical University of Lodz, University Clinical Hospital No 2, Łódź, Poland

author

Ewa Slominska

• Department of Biochemistry, Medical University of Gdansk, Gdańsk, Poland

author

Ewa Polakowska

• Department of Pediatrics, Medical University of Lodz, Łódź, Poland

author

Wojciech Mlynarski

• Department of Pediatrics, Medical University of Lodz, Łódź, Poland

author

Cezary Watala

• Department of Haemostasis and Haemostatic Disorders, Chair of Laboratory Diagnostics, Medical University of Lodz, University Clinical Hospital No 2, Łódź, Poland

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