Reference genes for gene expression studies on non-small cell lung cancer

• Authors: Peter Gresner , Jolanta Gromadzinska , Wojciech Wasowicz
• Languages of publication: EN

Abstracts

EN

Study Objective: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. Method: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. Results: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. Conclusion: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.

Keywords

EN

non-small cell lung cancer; reference genes; data normalization; real-time PCR; gene expression

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2009
• Volume: 56
• Issue: 2
• Pages: 307-316

Dates

published

2009

received

2009-03-28

revised

2009-06-08

accepted

2009-06-11

(unknown)

2009-06-18

Contributors

author

Peter Gresner

• Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland

author

Jolanta Gromadzinska

• Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland

author

Wojciech Wasowicz

• Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland

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