Specific binding sites for progesterone and 17β-estradiol in cells of Triticum aestivum L.

• Authors: Anna Janeczko , Bogusława Budziszewska , Andrzej Skoczowski , Małgorzata Dybała
• Languages of publication: EN

Abstracts

EN

The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [3H]17β-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17β-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.

Keywords

EN

vernalization; progesterone; radioligand binding assay; Triticum aestivum L.; 17β-estradiol

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 4
• Pages: 707-711

Dates

published

2008

received

2008-07-11

revised

2008-11-20

accepted

2008-11-25

(unknown)

2008-12-16

Contributors

author

Anna Janeczko

• Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland

author

Bogusława Budziszewska

• Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland

author

Andrzej Skoczowski

• Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland

author

Małgorzata Dybała

• Laboratory of Pharmacobiology, Department of Cytobiology and Histochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

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