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2008 | 55 | 4 | 693-699

Article title

Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load

Content

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Languages of publication

EN

Abstracts

EN
Epstein-Barr virus (EBV), a member of the family Herpesviridae, is widely spread in the human population and has the ability to establish lifelong latent infection. In immunocompetent individuals the virus reactivation is usually harmless and unnoticeable. In immunocompromised patients productive infection or type III latency may lead to EBV-associated post-transplant lymphoproliferative disorder (PTLD). The aim of our research was to investigate the utility of PCR-based methods in the diagnosis and monitoring of EBV infections in bone marrow transplant recipients. Thirty-eight peripheral blood leukocyte samples obtained from 16 patients were analysed, in which EBV DNA was confirmed by PCR. We used semi-quantitative PCR to estimate the viral load and reverse-transcription PCR (RT-PCR) to differentiate between latent and productive EBV infection. In 14 patients we confirmed productive viral infection. We observed a correlation between higher number of EBV genome copies and the presence of transcripts specific for type III latency as well as clinical symptoms.

Year

Volume

55

Issue

4

Pages

693-699

Physical description

Dates

published
2008
received
2008-04-27
revised
2008-09-16
accepted
2008-11-03
(unknown)
2008-11-17

Contributors

  • Department of Virology, Chair of Microbiology, Jagiellonian University Medical Collage, Kraków, Poland
author
  • Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
author
  • Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Chair and Department of Haematology, Jagiellonian University Medical Collage, Kraków, Poland
  • Chair and Department of Haematology, Jagiellonian University Medical Collage, Kraków, Poland
  • Department of Virology, Chair of Microbiology, Jagiellonian University Medical Collage, Kraków, Poland

References

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Document Type

Publication order reference

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bwmeta1.element.bwnjournal-article-abpv55p693kz
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