The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants

• Authors: Agnieszka Dzikiewicz-Krawczyk , Paulina Piontek , Zofia Szweykowska-Kulińska , Artur Jarmołowski
• Languages of publication: EN

Abstracts

EN

In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.

Keywords

EN

nuclear cap-binding protein complex; alternative splicing; mRNA surveillance; NMD; premature terminatin codon; nonsense-mediated mRNA decay

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 4
• Pages: 825-828

Dates

published

2008

received

2008-06-26

revised

2008-09-18

accepted

2008-11-17

(unknown)

2008-12-16

Contributors

author

Agnieszka Dzikiewicz-Krawczyk

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Paulina Piontek

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Zofia Szweykowska-Kulińska

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Artur Jarmołowski

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

References

• Arciga-Reyes L, Wootton L, Kieffer M, Davies B (2006) Plant J 47: 480-489.
• Chiu SY, Lejeune F, Ranganathan AC, Maquat LE (2004) Genes Dev 18: 745-754.
• Conti E, Izaurralde E (2005) Curr Opin Cell Biol 17: 316-325.
• Green RE (2003) Bioinformatics 19 (Suppl 1): i118-i121.
• Hori K, Watanabe Y (2005) Plant J 43: 530-540.
• Hosoda N, Kim YK, Lejeune F, Maquat LE (2005) Nat Struct Mol Biol 12: 893-901.
• Hugouvieux V, Kwak JM, Schroeder JI (2001) Cell 106: 477-487.
• Ishigaki Y, Li X, Serin G, Maquat LE (2001) Cell 106: 607-617.
• Isshiki M, Yamamoto Y, Satoh H, Shimamoto K (2001) Plant Physiol 125: 1388-1395.
• Kertesz S, Kerenyi Z, Merai Z, Bartos I, Palfy T, Barta E, Silhavy D (2006) Nucleic Acids Res 34: 6147-6157.
• Kmieciak M, Simpson CG, Lewandowska D, Brown JWS, Jarmolowski A (2002) Gene 283: 171-183.
• Lejeune F, Maquat LE (2005) Curr Opin Cell Biol 17: 309-315.
• Maquat LE (2004) Nat Rev Mol Cell Biol 5: 89-99.
• Papp I, Mur LA, Dalmadi A, Dulai S, Koncz C (2004) Plant Mol Biol 55: 679-686.
• Petracek ME, Nuygen T, Thompson WF, Dickey LF (2000) Plant J 21: 563-569.
• van Hoof A, Green PJ (1996) Plant J 10: 415-424.
• Wagner E, Lykke-Andersen J (2002) J Cell Sci 115: 3033-3038.
• Wang BB, Brendel V (2006) Proc Natl Acad Sci USA 103: 7175-7180.
• Zhang S, Ruiz-Echevarria MJ, Quan Y, Peltz SW (1995) Mol Cell Biol 15: 2231-2244.