SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues

• Authors: Katarzyna Bąbol-Pokora , Jarosław Berent
• Languages of publication: EN

Abstracts

EN

SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism® 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.

Keywords

EN

formalin-fixed paraffin-embedded tissues; SNP; minisequencing; low copy number DNA

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 4
• Pages: 815-819

Dates

published

2008

received

2008-04-27

revised

2008-07-08

accepted

2008-10-08

(unknown)

2008-11-4

Contributors

author

Katarzyna Bąbol-Pokora

• Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland

author

Jarosław Berent

• Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland

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