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2008 | 55 | 3 | 499-506

Article title

Effects of inhibitor of Src kinases, SU6656, on differentiation of megakaryocytic progenitors and activity of α1,6-fucosyltransferase

Content

Title variants

Languages of publication

EN

Abstracts

EN
α1,6-fucosyltransferase (FUT8) attaches fucose residues via an α1,6 linkage to the innermost N-acetylglucosamine residue of N-linked glycans. Glycans with this type of structure are present in GpIIb/GpIIIa complex (CD41a) which is present on megakaryocytes (Mks) and platelets. CD41a is the earliest marker of megakaryocytopoiesis. The aim of this study was to analyse the morphology, phenotype, ploidy level and activity of FUT8 during induced differentiation/maturation of Mk progenitor cells in ex vivo culture. We used SU6656, a selective inhibitor of Src tyrosine kinases, as differentiation-inducing agent for Mks. The addition of SU6656 to the culture system of megakaryocytic progenitors from cord blood CD34+ cells and Meg-01 cell line induced their maturation towards later stages of Mk differentiation with increased activity of FUT8. We suggest FUT8 as a candidate for an early marker of differentiation and possibly of the ploidy level of Mks. We confirm a special status of FUT8 in megakaryocytopoiesis.

Year

Volume

55

Issue

3

Pages

499-506

Physical description

Dates

published
2008
received
2008-03-18
revised
2008-09-06
accepted
2008-09-11
(unknown)
2008-09-15

Contributors

  • Deparment of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland
  • Deparment of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland
  • Department of Cell Biology, National Medicines Institute, Warszawa, Poland
  • Deparment of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv55p499kz
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