Dynamics of estrogen-induced oxidative stress

• Authors: Jarek Kobiela , Tomasz Stefaniak , Jacek Krajewski , Beata Kalinska-Blach , Dorota Zurawa-Janicka , Andrzej Lachinski , Daniel Gackowski , Ryszard Olinski , Jerzy Nowak , Narcyz Knap , Barbara Lipinska , Zbigniew Sledzinski , Michal Wozniak
• Languages of publication: EN

Abstracts

EN

The objective of this study was to assess the dynamics of oxidative damage to cellular macromolecules such as proteins, lipids and DNA under conditions of oxidative stress triggering early stages of estrogen-dependent carcinogenesis. A rodent model of carcinogenesis was used. Syrian hamsters were sacrificed after 1, 3, 5 h and one month from the initial implantation of estradiol. Matching control groups were used. Kidneys as target organs for estradiol-mediated oxidative stress were excised and homogenized for biochemical assays. Subcellular fractions were isolated. Carbonyl groups (as a marker of protein oxidation) and lipid hydroxyperoxides were assessed. DNA was isolated and 8-oxodGuo was assessed. Electron paramagnetic resonance spectroscopy was used to confirm the results for lipid peroxidation. Exposition to estradiol in the rodent model leads to damage of macromolecules of the cell, including proteins and DNA, but not lipids. Proteins appear to be the primary target of the damage but are closely followed by DNA. It has previously been speculated that protein peroxides can increase DNA modifications. This time sequence was observed in our study. Nevertheless, the direct relation between protein and DNA damage still remains unsolved.

Keywords

EN

8-oxodGuo; estradiol; protein oxidation; carcinogenesis; DNA damage; lipid peroxidation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 2
• Pages: 289-295

Dates

published

2007

received

2006-10-03

accepted

2007-04-12

revised

2007-04-12

(unknown)

2007-05-15

Contributors

author

Jarek Kobiela

• Department of Medical Chemistry, Medical University of Gdansk, Gdańsk, Poland

author

Tomasz Stefaniak

• Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, Gdańsk, Poland

author

Jacek Krajewski

• Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, Gdańsk, Poland

author

Beata Kalinska-Blach

• Department of Medical Chemistry, Medical University of Gdansk, Gdańsk, Poland

author

Dorota Zurawa-Janicka

• Department of Biochemistry, University of Gdansk, Gdańsk, Poland

author

Andrzej Lachinski

• Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, Gdańsk, Poland

author

Daniel Gackowski

• Department of Clinical Biochemistry, Collegium Medicum, Nicolaus Copernicus University, Toruń, Poland

author

Ryszard Olinski

• Department of Clinical Biochemistry, Collegium Medicum, Nicolaus Copernicus University, Toruń, Poland

author

Jerzy Nowak

• Department of Biophysics, Medical University of Gdansk, Gdańsk, Poland

author

Narcyz Knap

• Department of Medical Chemistry, Medical University of Gdansk, Gdańsk, Poland

author

Barbara Lipinska

• Department of Biochemistry, University of Gdansk, Gdańsk, Poland

author

Zbigniew Sledzinski

• Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, Gdańsk, Poland

author

Michal Wozniak

• Department of Medical Chemistry, Medical University of Gdansk, Gdańsk, Poland

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