Plasma membrane homing of tissue nonspecific alkaline phosphatase under the influence of 3-hydrogenkwadaphnin, an antiproliferative agent from Dendrostellera lessertii

• Authors: Akram Sadeghirizi , Razieh Yazdanparast
• Languages of publication: EN

Abstracts

EN

Several mammalian enzymes are anchored to the outer surface of the plasma membrane by a covalently attached glycosylphosphatidylinositol (GPI) structure. These include acetylcholinesterase, alkaline phosphatase (AP) and 5'-nucleotidase among other enzymes. Recently, it has been reported that these membrane enzymes can be released into the serum by the GPI-dependent phospholipase D under various medical disturbances such as cancer and/or by chemical and physical manipulation of the biological systems. Treatment of MCF-7 cells with two consecutive effective concentrations of 3-hydrogenkwadaphnin (3-HK, 3 nM) for 48 h enhanced membrane AP activity by almost 330% along with a 40% reduction in the AP activity of the cell culture medium. In addition, our data indicate that 3-HK is capable of inducing mainly the tissue-nonspecific alkaline phosphatase (TNAP) isoenzyme, along with enhancing its thermostability. These findings, besides establishing a correlation between the antiproliferative activity of 3-HK and the extent of plasma membrane AP activity, might assist in the development of new diagnostic tools for following cancer medical treatments.

Keywords

EN

alkaline phosphatase; anti-proliferative; Dendrostellera lessertii

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 2
• Pages: 323-329

Dates

published

2007

received

2006-06-03

revised

2006-11-20

accepted

2007-04-24

(unknown)

2007-05-23

Contributors

author

Akram Sadeghirizi

• Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran, Teheran, Iran

author

Razieh Yazdanparast

• Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran, Teheran, Iran

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