Attaching a spin to a protein - site-directed spin labeling in structural biology

• Authors: Aleksander Czogalla , Aldona Pieciul , Adam Jezierski , Aleksander F Sikorski
• Languages of publication: EN

Abstracts

EN

Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.

Keywords

EN

protein structure; site-directed spin labeling; electron paramagnetic resonance (EPR) spectroscopy

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 2
• Pages: 235-244

Dates

published

2007

received

2007-04-19

revised

2007-05-29

accepted

2007-06-11

(unknown)

2007-06-14

Contributors

author

Aleksander Czogalla

• Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Aldona Pieciul

• Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Adam Jezierski

• Faculty of Chemistry, University of Wrocław, Wrocław, Poland

author

Aleksander F Sikorski

• Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

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