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2007 | 54 | 1 | 107-112

Article title

The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene

Content

Title variants

Languages of publication

EN

Abstracts

EN
The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expression of the chloramphenicol acetyltransferase reporter gene in testes of transgenic mice. However, in spite of the drastically reduced overall activity, the stage-specific expression pattern of the transgene was preserved after removal of these sequences. We have also shown that GC-box located downstream of the box B (approximately 210 bp upstream of the starting ATG codon) is indispensable for efficient expression of the Hsp70.2/Hst70 gene promoter in spermatogenic cells. The GC-box specifically binds proteins present in nuclear extracts from testes (putatively Sp1-like factors). A change in the pattern of such GC-box-interacting factors corresponds to activation of the Hsp70.2/Hst70 gene, confirming the importance of this regulatory element.

Year

Volume

54

Issue

1

Pages

107-112

Physical description

Dates

published
2007
received
2006-08-29
revised
2006-12-18
accepted
2007-01-12
(unknown)
2007-02-20

Contributors

  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland
author
  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland
  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland
  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland
  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland
  • Department of Tumor Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Branch, Gliwice, Gliwice, Poland

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv54p107kz
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