Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.

• Authors: Kacper Kazimierczuk , Lucyna Cova , Benedicte Ndeboko , Urszula Szczyrk , Aneta Targosz , Tomasz Brzozowski , Agnieszka Sirko
• Languages of publication: EN

Abstracts

EN

Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.

Keywords

EN

urease; antibody; antigen; IgY; DNA immunization; Helicobacter pylori

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 1
• Pages: 261-266

Dates

published

2005

received

2005-01-12

accepted

2005-02-10

Contributors

author

Kacper Kazimierczuk

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Lucyna Cova

• INSERM U271, Lyon Cedex 03, France

author

Benedicte Ndeboko

• INSERM U271, Lyon Cedex 03, France

author

Urszula Szczyrk

• Department of Physiology, Medical College of Jagiellonian University, Kraków, Poland

author

Aneta Targosz

• Department of Physiology, Medical College of Jagiellonian University, Kraków, Poland

author

Tomasz Brzozowski

• Department of Physiology, Medical College of Jagiellonian University, Kraków, Poland

author

Agnieszka Sirko

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

References

• Blanchard TG, Czinn SJ, Maurer R, Thomas WD, Soman G, Nedrud JG. (1995) Infect Immun.; 63: 1394-9.
• Brodzik R, Gaganidze D, Hennig J, Muszynska G, Koprowski H, Sirko A. (2000a) In Food Biotechnology, vol. 17, eds. Bielecki S, Tramper J, Polak J, pp 35-42. Elsevier, Amsterdam, Netherlands.
• Brodzik R, Koprowski H, Yusibov V, Sirko A. (2000b) Cell Mol Biol Lett.; 5: 357-66.
• Casswall TH, Nilsson HO, Bjorck L, Sjostedt S, Xu L, Nord CK, Boren T, Wadstrom T, Hammarstrom L. (2002) Scand J Gastroenterol.; 37: 1380-5.
• Hirota K, Nagata K, Norose Y, Futagami S, Nakagawa Y, Senpuku H, Kobayashi M, Takahashi H. (2001) Infect Immun.; 69: 6597-603.
• Houimel M, Corthesy-Theulaz I, Fisch I, Wong C, Corthesy B, Mach J, Finnern R. (2001) Tumour Biol.; 22: 36-44.
• Hu LT, Mobley HL. (1993) Infect Immun.; 61: 2563-9.
• Marnila P, Rokka S, Rehnberg-Laiho L, Karkkainen P, Kosunen TU, Rautelin H, Hanninen ML, Syvaoja EL, Korhonen H. (2003) Helicobacter.; 8: 192-201.
• Prinz C, Hafsi N, Voland P. (2003) Trends Microbiol.; 11: 134-8.
• Reiche N, Jung A, Brabletz T, Vater T, Kirchner T, Faller G. (2002) Infect Immun.; 70: 4158-64.
• Rollier C, Charollois C, Jamard C, Trepo C, Cova L. (2000) J Virol.; 74: 4908-11.
• Ruggiero P, Peppoloni S, Berti D, Rappuoli R, Giudice GD. (2002) Expert Opin Investig Drugs.; 11: 1127-38.
• Sambrook J, Fritsch EF, Maniatis T. (1989) Molecular Cloning. A Laboratory Manual. 2edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
• Shimamoto C, Tokioka S, Hirata I, Tani H, Ohishi H, Katsu K. (2002) Hepatogastroenterology.; 49: 709-14.
• Shin JH, Yang M, Nam SW, Kim JT, Myung NH, Bang WG, Roe IH. (2002) Clin Diagn Lab Immunol.; 9: 1061-6.
• Shin JH, Nam SW, Kim JT, Yoon JB, Bang WG, Roe IH. (2003) J Med Microbiol.; 52: 217-22.
• Thomas JE, Whatmore AM, Kehoe MA, Skillen AW, Barer MR. (1992) J Clin Microbiol.; 30: 1338-40.