Analysis of ligand binding process using binding capacity concept.

• Authors: A K Bordbar , Z Saadati , N Sohrabi
• Languages of publication: EN

Abstracts

EN

Binding capacity is the homotropic second derivative of the binding potential with respect to the chemical potential of the ligand. It provides a measure of steepness of the binding isotherm and represents the extent of cooperativity. In the present study, the shape of the binding capacity curve for various systems was investigated and the relation between binding capacity and the extent of cooperativity examined. In this regard, a novel linear graphical method was introduced for binding data analysis. The stoichiometry of binding and the extent of cooperativity can be determined by this method. This method has been successfully applied to various systems such as binding of oxygen to hemoglobin, warfarin to human serum albumin and dodecyltrimethylammonium bromide to α-amylase.

Keywords

EN

cooperativity; ligand binding; binding capacity; stoichiometry of binding

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 4
• Pages: 963-970

Dates

published

2004

received

2003-10-10

revised

2004-06-28

accepted

2004-07-22

Contributors

author

A K Bordbar

• Department of Chemistry, Isfahan University, Isfahan, Iran

author

Z Saadati

• Department of Chemistry, Islamic Azad University, Omidiye Unit, Omidiye, Iran

author

N Sohrabi

• Department of Chemistry, Isfahan University, Isfahan, Iran

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