Benzodiazepine binding to mitochondrial membranes of the amoeba Acanthamoeba castellanii and the yeast Saccharomyces cerevisiae.

• Authors: Malgorzata Slocinska , Adam Szewczyk , Lilla Hryniewiecka , Hanna Kmita
• Languages of publication: EN

Abstracts

EN

Benzodiazepine binding sites were studied in mitochondria of unicellular eukaryotes, the amoeba Acathamoeba castellanii and the yeast Saccharomyces cerevisiae, and also in rat liver mitochondria as a control. For that purpose we applied Ro5-4864, a well-known ligand of the mitochondrial benzodiazepine receptor (MBR) present in mammalian mitochondria. The levels of specific [3H]Ro5-4864 binding, the dissociation constant (KD) and the number of [3H]Ro5-4864 binding sites (Bmax) determined for fractions of the studied mitochondria indicate the presence of specific [3H]Ro5-4864 binding sites in the outer membrane of yeast and amoeba mitochondria as well as in yeast mitoplasts. Thus, A. castellanii and S. cerevisiae mitochondria, like rat liver mitochondria, contain proteins able to bind specifically [3H]Ro5-4864. Labeling of amoeba, yeast and rat liver mitochondria with [3H]Ro5-4864 revealed proteins identified as the voltage dependent anion selective channel (VDAC) in the outer membrane and adenine nucleotide translocase (ANT) in the inner membrane. Therefore, the specific MBR ligand binding is not confined only to mammalian mitochondria and is more widespread within the eukaryotic world. However, it can not be excluded that MBR ligand binding sites are exploited efficiently only by higher multicellular eukaryotes. Nevertheless, the MBR ligand binding sites in mitochondria of lower eukaryotes can be applied as useful models in studies on mammalian MBR.

Keywords

EN

Saccharomyces cerevisiae; Acathamoeba castellanii; Ro5-4864; mitochondrial benzodiazepine receptor

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 4
• Pages: 953-962

Dates

published

2004

received

2004-03-09

revised

2004-07-01

accepted

2004-10-28

Contributors

author

Malgorzata Slocinska

• Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

author

Adam Szewczyk

• Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Warszawa, Poland

author

Lilla Hryniewiecka

• Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

author

Hanna Kmita

• Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

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