Enzymatic redox reactions of the explosive 4,6-dinitrobenzofuroxan (DNBF): implications for its toxic action.

• Authors: Aušra Nemeikaitė-Čėnienė , Jonas Šarlauskas , Lina Misevièienė , Žilvinas Anusevièius , Audronė Marozienė , Narimantas Čėnas
• Languages of publication: EN

Abstracts

EN

With an aim to understand the toxicity mechanisms of the explosive 4,6-dinitro- benzofuroxan (DNBF), we studied its single-electron reduction by NADPH:cytochrome P450 reductase and ferredoxin:NADP+ reductase, and two- electron reduction by DT-diaphorase and Enterobacter cloacae nitroreductase. The enzymatic reactivities of DNBF and another explosive 2,4,6-trinitrotoluene (TNT) were similar, except for the much lower reactivity of DNBF towards nitroreductase. DNBF was less cytotoxic in FLK cells than TNT. However, their action shared the same mechanisms, oxidative stress and activation by DT-diaphorase. The lower cytotoxicity of DNBF may be explained by the negative electrostatic charge of its adduct with water which may impede cellular membrane penetration, and by the formation of its less reactive adducts with intracellular reduced glutathione.

Keywords

EN

cytotoxicity; nitroaromatic explosives; oxidative stress

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 4
• Pages: 1081-1086

Dates

published

2004

received

2004-04-30

revised

2004-07-02

accepted

2004-10-23

Contributors

author

Aušra Nemeikaitė-Čėnienė

• Institute of Immunology, Molėtų Pl. 29, Vilnius 2021, Lithuania

author

Jonas Šarlauskas

• Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania

author

Lina Misevièienė

• Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania

author

Žilvinas Anusevièius

• Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania

author

Audronė Marozienė

• Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania

author

Narimantas Čėnas

• Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania

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