Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induce PAL activity in Rubus cells.

• Authors: Mihai Nita-Lazar , Alain Heyraud , Claude Gey , Isabelle Braccini , Yvette Lienart
• Languages of publication: EN

Abstracts

EN

Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. Our results indicate that a disaccharide fraction containing the following structure: α-D-mannopyranosyl (1→2)α/β-D-glucopyranosyl and α-D-mannopyranosyl (1→x) inositol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar concentration. The disaccharides were isolated by size-exclusion chromatography directly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry.

Keywords

EN

oligosaccharide elicitors; Rubus fructicosus; phenylalanine ammonia lyase; Fusarium oxysporum

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 625-634

Dates

published

2004

received

2004-02-26

revised

2004-06-17

accepted

2004-07-20

Contributors

author

Mihai Nita-Lazar

• Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France

author

Alain Heyraud

• Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France

author

Claude Gey

• Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France

author

Isabelle Braccini

• Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France

author

Yvette Lienart

• Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France

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