Tissue-specific effect of refeeding after short- and long-term caloric restriction on malic enzyme gene expression in rat tissues.

• Authors: Ewa Stelmanska , Justyna Korczynska , Julian Swierczynski
• Languages of publication: EN

Abstracts

EN

Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced in rats maintained on restricted diet for 30 days than for 3 days. The changes in ME gene expression resembled the changes in the content of SREBP-1 in white adipose tissue. A similar increase of serum insulin concentration was observed in all groups at different degrees of caloric restriction and refed ad libitum for 48 h. Caloric restriction and refeeding caused on increase of ME activity also in brown adipose tissue (BAT) and liver. However, in liver a significant increase of ME activity was found only in rats maintained on the restricted diet for 30 days. No significant changes after caloric restriction and refeeding were found in heart, skeletal muscle, kidney cortex, and brain. These data indicate that the increase of ME gene expression after caloric restriction/refeeding occurs only in lipogenic tissues. Thus, one can conclude that caloric restriction/refeeding increases the enzymatic capacity for fatty acid biosynthesis.

Keywords

EN

white adipose tissue (rat); caloric restriction; malic enzyme; refeeding

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 805-814

Dates

published

2004

received

2003-11-19

revised

2004-02-20

accepted

2004-03-06

Contributors

author

Ewa Stelmanska

• Department of Biochemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Justyna Korczynska

• Department of Biochemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Julian Swierczynski

• Department of Biochemistry, Medical University of Gdańsk, Gdańsk, Poland

References

• Anson RM, Guo Z, de Cabo R, Iyun T, Rios M, Hagepanos A, Ingram DK, Lane MA, Mattson MP. (2003) Intermittent fasting dissociates beneficial effects of dietary restriction on glucose metabolism and neuronal resistance to injury from calorie intake. Proc Natl Acad Sci USA.; 100: 6216-20.
• Brdiczka D, Pette D. (1971) Intra- and extramitochondrial isozymes of (NADP) malate dehydrogenase. Eur J Biochem.; 19: 546-51.
• Brochu M, Poehlman ET, Ades PA. (2000) Obesity, body fat distribution, and coronary artery disease. J Cardiopulm Rehabil.; 20: 96-108.
• Cao SX, Dhahbi JM, Mote PL, Spindler SR. (2001) Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci USA.; 98: 10630-5.
• Chomczynski P, Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem.; 162: 156-9.
• Dhahbi JM, Mote PL, Wingo J, Tillman JB, Walford RL, Spindler SR. (1999) Calories and aging alter gene expression for gluconeogenic, glycolytic, and nitrogen-metabolizing enzymes. Am J Physiol Endocrinol Metab.; 277: E352-60.
• Dhahbi JM, Mote PL, Wingo J, Rowley BC, Cao SX, Walford RL, Spindler SR. (2001) Caloric restriction alters the feeding response of key metabolic enzymes genes. Mech Ageing Dev.; 122: 1033-48.
• Dhahbi JM, Mote PL, Cao SX, Spindler SR. (2003) Hepatic gene expression profiling of streptozotocin-induced diabetes. Diabetes Technol Ther., 5: 411-20.
• Frenkel R. (1975) Regulation and physiological functions of malic enzymes. Curr Top Cell Regul.; 9: 157-81.
• Gondret F, Lebas F, Bonneau M. (2000) Restricted feed intake during fattening reduces intramuscular lipid deposition without modifying muscle fiber characteristics in rabbits. J Nutr.; 130: 228-33.
• Goodrick GK, Foreyt JP. (1991) Why treatments for obesity don't last. J Am Diet Assoc.; 91: 1243-7.
• Karbowska J, Kochan Z, Zelewski L, Swierczynski J. (1999) Tissue specific effect of clofibrate on rat lipogenic enzyme gene expression. Eur J Pharmacol.; 370: 329-36.
• Kochan Z, Karbowska J, Swierczynski J. (1997) Unusual increase of lipogenesis in rat white adipose tissue after multiple cycles of starvation-refeeding. Metabolism.; 46: 10-7.
• Korczynska J, Stelmanska E, Nieweglowski T, Szolkiewicz M, Rutkowski B, Swierczynski J. (2000) Effect of clofibrate on plasma lipid concentration and malic enzyme gene expression in rats with experimental chronic renal failure. Pol J Pharmacol.; 52: 291-7.
• Korczynska J, Stelmanska E, Swierczynski J. (2003) Differential effect of long term food restriction on fatty acid synthase and leptin genes expression in rat white adipose tissue. Horm Metab Res.; 35: 593-7.
• Lebovitz HE. (1999) Type 2 diabetes: an overview. Clin Chem.; 45: 1339-45.
• Levi F. (1999) Cancer prevention: epidemiology and perpectives. Eur J Cancer.; 35: 1912-24.
• Masoro EJ. (1995) Dietary restriction. Exp Gerontol.; 30: 291-8.
• McNeel RL, Mersmann HJ. (2000) Nutritional deprivation reduces the transcripts for transcription factors and adipocyte-characteristic proteins in porcine adipocytes. J Nutr Biochem.; 11: 139-46.
• Osborne TF. (2000) Sterol regulatory element-binding proteins (SREBPs): key regulators of nutritional homeostasis and insulin action. J Biol Chem.; 275: 32379-82.
• Roth GS, Ingram DK, Lane MA. (2001) Caloric restriction in primates and relevance to humans. Ann N Y Acad Sci.; 928: 305-15.
• Shimokawa I, Higami Y, Tsuchiya T, Otani H, Komatsu T, Chiba T, Yamaza H. (2003) Life span extension by reduction of the growth hormone-insulin-like growth-1 axis: relation to caloric restriction. FASEB J.; 17: 1108-9.
• Spindler SR. (2003) Caloric restriction, longevity and the search for authentic anti-aging drugs. In: Kinney B, Carraway J, eds, Quality Medical Publishing, Inc., St Louis.
• Trayhurn P, Duncan JS, Rayner DV. (1995) Acute cold-induced suppression of ob (obese) gene expression in white adipose tissue of mice: mediation by the sympathetic system. Biochem J.; 311: 729-33.
• Vecchini A, Ceccarelli V, Orvietani P, Caligiana P, Susta F, Binaglia L, Nocentini G, Riccardi C, Di Nardo P. (2003) Enhanced expression of lipogenic enzymes in an animal model of sedentariness. J Lipid Res.; 44: 696-704.
• Weindruch R, Walford RL. (1988) The Retardation of Aging and Disease by Dietary Restriction. Charles C Thomas Publisher, Springfield, IL.
• Weindruch R, Sohal RS. (1997) Seminars in medicine of the Beth Israel Deaconess Medical Center. Caloric intake and aging. N Engl J Med.; 337: 986-94.
• Weindruch R, Kayo T, Lee Ch-K, Prolla TA. (2001) Microarray profilling of gene expression in aging and its alteration by caloric restriction in mice. J Nutr.; 131: 918S-23S.
• Zelewski M, Swierczynski J. (1990) Comparative studies on lipogenic enzyme activities in brown adipose tissue and liver of rat during starvation-refeeding transition and cold exposure. Comp Biochem Physiol B.; 97: 59-63.