Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD(P)H:quinone reductase and apoptosis in human lymphoblastoid cells.

• Authors: Irena Misiewicz , Katarzyna Skupińska , Elżbieta Kowalska , Jan Lubiński , Teresa Kasprzycka-Guttman
• Languages of publication: EN

Abstracts

EN

The effect of sulforaphane on human lymphoblastoid cells originating from a patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance of chemopreventive activity. In our study, changes in cell growth, induction of apoptosis and phase 2 enzymes as well as glutathione level were examined. Apoptosis was tested by confocal microscopy at three stages: change in mitochondrial membrane potential, caspase activation and phosphatidylserine externalization. We show that SFN increases the activity of the detoxification system: it increases quinone reductase activity at low concentration (0.5-1 μM) and raises glutathione level in a dose-dependent manner. At higher doses (2.5-10 μM) sulforaphane is a cell growth modulator, as it caused cell growth cessation (IC50 = 3.875 μM), and apoptosis inducer. The results obtained suggest that sulforaphane acts as a chemopreventive agent in human lymphoblastoid cells.

Keywords

EN

cancer chemoprevention; sulforaphane; lymphoblastoid cells

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 711-721

Dates

published

2004

received

2004-01-30

revised

2004-04-21

accepted

2004-05-07

Contributors

author

Irena Misiewicz

• Confocal Microscopy Laboratory, National Institute of Public Health, Warszawa, Poland

author

Katarzyna Skupińska

• Confocal Microscopy Laboratory, National Institute of Public Health, Warszawa, Poland

author

Elżbieta Kowalska

• Department of Genetics and Patomorphology, Pomeranian Academy of Medicine, Szczecin, Poland

author

Jan Lubiński

• Department of Genetics and Patomorphology, Pomeranian Academy of Medicine, Szczecin, Poland

author

Teresa Kasprzycka-Guttman

• Department of Chemistry, Warsaw University, Warszawa, Poland

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