Degradation of extracellular nucleotides and their analogs in HeLa and HUVEC cell cultures.

• Authors: Edyta Gendaszewska-Darmach , Maria Maszewska , Małgorzata Zakłos , Maria Koziołkiewicz
• Languages of publication: EN

Abstracts

EN

The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ATP and ADP in the HeLa epitheloid carcinoma and endothelial HUVEC cells cultures. Our studies have revealed that ecto-nucleotide pyrophosphatase (E-NPP) is one of the main enzymes involved in the extracellular degradation of ATP and other nucleotides in the HeLa cells. On the other hand, the ecto-ATPDase is responsible for the hydrolysis of extracellular nucleotides in human endothelial cell cultures, while the E-NPP-like enzymes of the HUVEC cells are not essential to this degradation. The concerted action of the aforementioned ecto-enzymes and nucleotide pyrophosphatase, 5'-nucleotidase and adenosine deaminase present in fetal bovine serum (FBS) supplied to the culture medium, results in partial or complete degradation of the phosphorothioate (ATPγS) and phosphonate analogs of adenosine nucleotides (α,β-methylene-ATP and β,γ-methylene-ATP) in the cell cultures. Only ADPβS appears to be resistant to these enzymes. The influence of some nucleotides and their analogs on the proliferation of the HeLa cells in presence or absence of FBS is also discussed.

Keywords

EN

nucleotide analogs; extracellular nucleotides; ectoenzymes; nucleotide pyrophosphatase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 4
• Pages: 973-984

Dates

published

2003

received

2003-10-03

revised

2003-11-14

Contributors

author

Edyta Gendaszewska-Darmach

• Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies Polish Academy of Sciences, Łódź, Poland

author

Maria Maszewska

• Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies Polish Academy of Sciences, Łódź, Poland

author

Małgorzata Zakłos

• >Institute of Technical Biochemistry, Technical University of Łódź, Łódź, Poland

author

Maria Koziołkiewicz

• Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies Polish Academy of Sciences, Łódź, Poland

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