Comparative analysis of RCAS1 level in neoplasms and placenta.

• Authors: Lukasz Wicherek , Magdalena Dutsch , Pawel Mak , Marek Klimek , Jacek Skladzien , Adam Dubin
• Languages of publication: EN

Abstracts

EN

The tumor associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) expressed with high frequency in various cancer and trophoblast cells, inhibits growth of estrogen receptor-expressing cells and induces apoptosis. Because previous reports demonstrated RCAS1 presence only by non-quantitative immunocytochemistry methods, we decided to use a Western blotting with anti-RCAS1 monoclonal antibodies for estimation of the relative content of the tumor-associated antigen. One hundred tissue samples were assayed (neoplasms, chronic inflammatory diseases, healthy tissues, trophoblasts and placentas at term). RCAS1 was present in all neoplastic, placental and trophoblast tissue samples and its level in malignant samples was statistically significantly higher than in benign neoplasms. The amount of RCAS1 in chronic inflammations was also significantly increased in immune mediated diseases, like allergic nasal polyps and sarcoidosis. The RCAS1 protein was not revealed in healthy mucous membrane and in muscle tissues. The presented results suggest that RCAS1 might play an important role in tumor escape from host immunological surveillance and carry weight in the down regulation of the maternal immune response, thereby maintaining pregnancy.

Keywords

EN

EBAG9; RCAS1; immune escape; apoptotic cell death; fetal rejection

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 4
• Pages: 1187-1194

Dates

published

2003

received

2003-05-30

revised

2003-10-22

accepted

2003-11-28

Contributors

author

Lukasz Wicherek

• Department of Gynecology and Infertility Clinic, Jagiellonian University, Kraków, Poland

author

Magdalena Dutsch

• Department of Otolaryngology, Jagiellonian University, Kraków, Poland

author

Pawel Mak

• Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

author

Marek Klimek

• Department of Gynecology and Infertility Clinic, Jagiellonian University, Kraków, Poland

author

Jacek Skladzien

• Department of Otolaryngology, Jagiellonian University, Kraków, Poland

author

Adam Dubin

• Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

References

• Engelsberg A, Hermosilla R, Karsten U, Schulein R, Dorken B, Rehm A. (2003) The Golgi protein RCAS1 controls cell surface expression of tumor-associated O-linked glycan antigens. J Biol Chem.; 278: 22998-3007.
• Enjoji M, Nakashima M, Nishi H, Choi I, Oimomi H, Sugimoto R, Kotoh K, Taguchi K, Nakamuta M, Nawata H, Watanabe T. (2002) The tumor-associated antigen, RCAS1, can be expressed in immune-mediated diseases as well as in carcinomas of biliary tract. J Hepatol.; 36: 786-92.
• Kubokawa M, Nakashima M, Yao T, Ito KI, Harada N, Nawata H, Watanabe T. (2001) Aberrant intracellular localization of RCAS1 is associated with tumor progression of gastric cancer. Int J Oncol.; 19: 695-700.
• Matsushima T, Nakashima M, Oshima K, Abe Y, Nishimura J, Nawata H, Watanabe T, Muta K. (2001) Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells. Blood.; 98: 313-21.
• Nakashima M, Sonoda K, Watanabe T. (1999) Inhibition of cell growth and induction of apoptotic cell death by the human tumor-associated antigen RCAS1. Nat Med.; 5: 938-42.
• Ohshima K, Muta K, Nakashima M, Haraoka S, Tutiya T, Suzumiya J, Kawasaki C, Watanabe T, Kikuchi M. (2001a) Expression of human tumor-associated antigen RCAS1 in Reed-Sternberg cells in association with Epstein-Barr virus infection: a potential mechanism of immune evasion. Int J Cancer.; 93: 91-6.
• Ohshima K, Nakashima M, Sonoda K, Kikuchi M, Watanabe T. (2001b) Expression of RCAS1 and FasL in human trophoblasts and uterine glands during pregnancy: the possible role in immune privilege. Clin Exp Immunol.; 123: 481-6.
• Oshikiri T, Hida Y, Miyamoto M, Hashida H, Katoh K, Suzuoki M, Nakakubo Y, Hiraoka K, Shinohara T, Itoh T, Kondo S, Katoh H. (2001) RCAS1 as a tumour progression marker: an independent negative prognostic factor in gallbladder cancer. Br J Cancer.; 85: 1922-7.
• Rousseau J, Tetu B, Caron D, Malenfant P, Cattaruzzi P, Audette M, Doillon C, Tremblay JP, Guerette B. (2002) RCAS1 is associated with ductal breast cancer progression. Biochem Biophys Res Commun.; 293: 1544-9.
• Schagger H, von Jagow G. (1987) Tricine-sodium dodecyl sulphate polyacrylamide gel electrophoresis for separation of proteins in the range from 1 kDa to 100 kDa. Anal Biochem.; 166: 368-79.
• Sonoda K, Kaku T, Hirakawa T, Kobayashi H, Amada S, Sakai K, Nakashima M, Watanabe T, Nakano H. (2000) The clinical significance of tumor-associated antigen RCAS1 expression in the normal, hyperplastic, and malignant uterine endometrium. Gynecol Oncol.; 79: 424-9.
• Suzuki T, Inoue S, Kawabata W, Akahira J, Moriya T, Tsuchiya F, Ogawa S, Muramatsu M, Sasano H. (2001) EBAG9/RCAS1 in human breast carcinoma: a possible factor in endocrine-immune interactions. Br J Cancer.; 85: 1731-7.
• Tsuneizumi M, Emi M, Nagai H, Harada H, Sakamoto G, Kasumi F, Inoue S, Kazui T, Nakamura Y. (2001) Overrepresentation of the EBAG9 gene at 8q23 associated with early-stage breast cancers. Clin Cancer Res.; 7: 3526-32.
• Umeoka K, Sanno N, Oyama K, Tahara S, Kurotani R, Ikuyama S, Nakashima M, Watanabe T, Osamura RY, Teramoto A. (2001) Immunohistochemical analysis of RCAS1 in human pituitary adenomas. Mod Pathol.; 14: 1232-6.
• Watanabe T, Inoue S, Hiroi H, Orimo A, Kawashima H, Muramatsu M. (1998) Isolation of estrogen-responsive genes with a CpG island library. Mol Cell Biol.; 18: 442-9.
• Wicherek L, Dutsch M, Mak P, Klimek M. (2003a) The presence of RCAS1 in pregnant uterus with concomitant ovarian cancer. Ginekol Prakt.; 72: 8-10.
• Wicherek L, Dutsch M, Mak P, Klimek M. (2003b) The presence of RCAS1/EBAG9 in placenta at term. Ginekol Prakt.; 74: 20-2.
• Winkler M, Fischer DC, Ruck P, Marx T, Kaiserling A, Oberpichler A, Tshesche H, Rath W. (1999) Parturition at term: parallel increases of interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment. Hum Reprod.; 14: 1096-1110.