Selective splitting of 3'-adenylated dinucleoside polyphosphates by specific enzymes degrading dinucleoside polyphosphates.

• Authors: Andrzej Guranowski , Antonio Sillero , María Antonia Günther Sillero
• Languages of publication: EN

Abstracts

EN

Several 3'-[32P]adenylated dinucleoside polyphosphates (NpnN'p*As) were synthesized by the use of poly(A) polymerase (Sillero MAG et al., 2001, Eur J Biochem.; 268 : 3605-11) and three of them, ApppA[32P]A or ApppAp*A, AppppAp*A and GppppGp*A, were tested as potential substrates of different dinucleoside polyphosphate degrading enzymes. Human (asymmetrical) dinucleoside tetraphosphatase (EC 3.6.1.17) acted almost randomly on both AppppAp*A, yielding approximately equal amounts of pppA + pAp*A and pA + pppAp*A, and GppppGp*, yielding pppG + pGp*A and pG + pppGp*A. Narrow-leafed lupin (Lupinus angustifolius) tetraphosphatase acted preferentially on the dinucleotide unmodified end of both AppppAp*A (yielding 90% of pppA + pAp*A and 10 % of pA + pppAp*A) and GppppGp*A (yielding 89% pppG + pGp*A and 11% of pG + pppGp*A). (Symmetrical) dinucleoside tetraphosphatase (EC 3.6.1.41) from Escherichia coli hydrolyzed AppppAp*A and GppppGp*A producing equal amounts of ppA + ppAp*A and ppG + ppGp*A, respectively, and, to a lesser extent, ApppAp*A producing pA + ppAp*A. Two dinucleoside triphosphatases (EC 3.6.1.29) (the human Fhit protein and the enzyme from yellow lupin (Lupinus luteus)) and dinucleoside tetraphosphate phosphorylase (EC 2.7.7.53) from Saccharomyces cerevisiae did not degrade the three 3'-adenylated dinucleoside polyphosphates tested.

Keywords

EN

diadenosine triphosphate; diadenosine tetraphosphatase; diadenosine triphosphatase; 3'-adenylated dinucleoside polyphosphates; diadenosine tetraphosphate; diguanosine tetraphosphate

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 1
• Pages: 123-130

Dates

published

2003

received

2002-11-11

revised

2003-02-11

accepted

2003-02-18

Contributors

author

Andrzej Guranowski

• Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols UAM/CSIC, Facultad de Medicina, 28029 Madrid, Spain

author

Antonio Sillero

• Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols UAM/CSIC, Facultad de Medicina, 28029 Madrid, Spain

author

María Antonia Günther Sillero

• Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols UAM/CSIC, Facultad de Medicina, 28029 Madrid, Spain

References

• Abdelghany HM, Gasmi L, Cartwright JL, Bailey S, Rafferty JB, McLennan AG. (2001) Cloning, characterization and crystallization of a diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans. Biochim Biophys Acta.; 1550: 27-36.
• Bailey S, Sedelnikova S, Blackburn GM, Abdelghany HM, Baker PJ, McLennan AG, Rafferty JB. (2002) The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms. Structure.; 10: 589-600.
• Baraniak J, Wasilewska E, Korczynski D, Stec WJ. (1999) Diadenylated polyols as new non-isopolar analogues of diadenosine tri- and tetraphosphates. Tetrahedron Lett.; 40: 8603-6.
• Barnes LD, Garrison PN, Siprashvili Z, Guranowski A, Robinson AK, Ingram S, Croce CM, Ohta M, Huebner K. (1996) Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry.; 35: 11529-35.
• Blackburn GM, Guo M-J, McLennan AG. (1992) Synthetic structural analogues of dinucleoside polyphosphates. In Ap4A and other dinucleoside polyphosphates. McLennan AG. Ed, pp 305-42. CRC Press, Boca Raton, FL.
• Garrison PN, Barnes LD. (1992) Determination of dinucleoside polyphosphates. In Ap4A and other dinucleoside polyphosphates. McLennan AG, ed, pp 29-61. CRC Press, Boca Raton, FL.
• Guranowski A, Blanquet S. (1985) Phosphorolytic cleavage of of diadenosine 5',5'''-P1,P4-tetraphosphate; properties of homogeneous diadenosine 5',5'''-P1,P4-tetraphosphate alpha,beta-phosphorylase from Saccharomyces cerevisiae. J Biol Chem.; 260: 3542-7.
• Guranowski A, Galbas M, Hartmann R, Justesen J. (2000) Selective degradation of 2'-adenylated diadenosine tri- and tetraphosphates, Ap3A and Ap4A, by two specific human dinucleoside polyphosphate hydrolases. Arch Biochem Biophys.; 373: 218-24.
• Guranowski A, Gunther Sillero MA, Sillero A. (1990) Firefly luciferase synthesizes P1,P4-bis(5'-adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphates. FEBS Lett.; 271: 215-8.
• Guranowski A, Jakubowski H, Holler E. (1983) Catabolism of diadenosine 5',5'''-P1,P4-tetraphosphate in prokaryotes; purification and properties of diadenosine 5',5'''-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12. J Biol Chem.; 258: 14784-9.
• Guranowski A, Sillero A. (1992) Enzymes cleaving dinucleoside polyphosphates. In Ap4A and other dinucleoside polyphosphates. McLennan AG, ed, pp 81-133. CRC Press, Boca Raton, FL.
• Guranowski A, Starzynska E, Bojarska E, Stepinski J, Darzynkiewicz D. (1996) Dinucleoside 5',5'''-P1,P3-triphosphate hydrolase from yellow lupin (Lupinus luteus) seeds; purification to homogeneity and hydrolysis of mRNA 5'-cap analogs. Protein Expr Purif.; 8: 416-22.
• Guranowski A. (2000) Specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates. Pharmacol Ther.; 87: 117-39.
• Jakubowski H, Guranowski A. (1983) Enzymes hydrolyzing ApppA and/or AppppA in higher plants; purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds. J Biol Chem.; 258: 9982-9.
• Lazewska D, Starzynska E, Guranowski A. (1993) Human placental (asymmetrical) diadenosine 5',5'''-P1,P4-tetraphosphate hydrolase: purification to homogeneity and some properties. Protein Expr Purif.; 4: 45-51.
• Liu H, Rodgers ND, Jiao X, Kiledjian M. (2002) The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases. EMBO J.; 21: 4699-708.
• Maksel D, Gooley PR, Swarbrick JD, Guranowski A, Gange Ch, Blackburn GM, Gayler KG. (2001) Characterization of active residues in diadenosine tetraphosphate hydrolase from Lupinus angustifolius. Biochem J.; 357: 399-405.
• McLennan AG. (2000) Dinucleoside polyphosphates - friend or foe? Pharmacol Ther.; 87: 73-89.
• Milone J, Wilusz J, Bellofatto V. (2002) Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts. Nucleic Acids Res.; 30: 4040-50.
• Ortiz B, Sillero A, Gunther Sillero MA. (1993) Specific synthesis of adenosine (5') tetraphospho (5') nucleoside and adenosine (5') oligophospho (5') adenosine (n>4) catalyzed by firefly luciferase. Eur J Biochem.; 212: 263-70.
• Plateau P, Blanquet S. (1992) Synthesis of NpnN' (n = 3 or 4) in vitro and in vivo. In Ap4A and other dinucleoside polyphosphates. McLennan AG, ed, pp 63-79. CRC Press, Boca Raton, FL.
• Schluter H, Gross I, Bachmann J, Kaufmann R, van der Giet M, Tepel M, Nofer JR, Assmann G, Karas M, Jankowski J, Zidek W. (1998) Adenosine (5') oligophospho-(5') guanosines and guanosine (5') oligophospho-(5') guanosines in human platelets. J Clin Invest.; 101: 682-8.
• Sekine M, Kadokura M, Satoh T, Seio K, Wada T. (1996) Chemical synthesis of a 5'-terminal TMG-capped triribonucleotide m32,2,7G5'pppAmpUmpA of U1 RNA.; J Org Chem.; 61: 4412-22.
• Sillero A, Gunther Sillero MA. (2000) Synthesis of dinucleoside polyphosphates catalyzed by luciferase and several ligases. Pharmacol Ther.; 87: 91-102.
• Sillero MAG, Madrid O, Zaera E, Sillero A. (1997) 2',3'-Dideoxynucleoside triphosphates (ddNTP) and di-2',3'-dideoxynucleoside tetraphosphates (ddNp4ddN) behave differently to the corresponding NTP and Np4N counterparts as substrates of firefly luciferase, dinucleoside tetraphosphatase and phosphodiesterases. Biochim Biophys Acta.; 1334: 191-9.
• Sillero MAG, Socorro S, Baptista MJ, del Valle M, de Diego A, Sillero A. (2001) Poly(A) polymerase from Escherichia coli adenylylates the 3'-hydroxyl residue of nucleosides, nucleoside 5'-phosphates and nucleoside(5')oligophospho(5')nucleosides (NpnN). Eur J Biochem.; 268: 3605-11.
• Stepinski J, Waddell C, Stolarski R, Darzynkiewicz E, Rhoads RE. (2001) Synthesis and properties of mRNAs containing the novel anti-reverse cap analogs 7-methyl(3'-O-methyl)GpppG and 7-methyl(3'-deoxy)GpppG. RNA.; 7: 1486-95.
• Swarbrick JD, Bashtannyk T, Maksel D, Zhang X-R, Blackburn GM, Gayler KR, Gooley PR. (2000) The three-dimensional structure of the nudix enzyme diadenosine tetraphosphate hydrolase from Lupinus angustifolius L. J Mol Biol.; 302: 1165-77.
• Thorne NMH, Hankin S, Wilkinson MC, Nunez C, Barraclough R, McLennan AG. (1995) Human diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase is a member of the MutT family of nucleotide pyrophosphatases. Biochem J.; 311: 717-21.
• Vallejo CG, Sillero MAG, Sillero A, (1974) Diguanosinetetraphosphate guanylohydrolase in Artemia salina. Biochim Biophys Acta.; 358: 117-25.
• Varnum JM, Baraniak J, Kaczmarek R, Stec WJ, Brenner Ch. (2001) Di- tri- and tetra-5'-O-phosphorothioadenosyl substituted polyols as inhibitors of Fhit: importance of the alpha-beta bridging oxygen and beta phosphorous replacement. BMC Chemical Biology 1: 3 (www.biomedcentral.com/1472-67691/1/3).