Detection of genotoxicity of atmospheric particles using a high-throughput microplate umu-test system.

• Authors: Kunihiro Funasaka , Masaaki Kitano , Akihiko Nakama , Taro Yoshikura , Yoshimitsu Oda
• Languages of publication: EN

Abstracts

EN

A previously developed and highly sensitive umu-microplate test system based on the nitroreductase- and O-acetyltransferase-overproducing strain Salmonella typhimurium NM3009 and the O-acetyltransferase-overproducing strain S. typhimurium NM2009 was applied to the detection of genotoxic activity in atmospheric particles in urban areas using a relatively small sample load. The results showed that the test system was able to detect slight increases in induced genotoxicity in atmospheric particles and that genotoxicity was detected mainly in the fine fraction but also partially in the coarse fraction. The present sensitive microplate test system has potential for application to the screening of various other environmental samples.

Keywords

EN

Salmonella typhimurium NM3009; umu-microplate test; nitroarenes; genotoxicity; atmospheric particles; Salmonella typhimurium NM2009

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 1
• Pages: 291-296

Dates

published

2003

received

2002-09-07

accepted

2003-01-31

Contributors

author

Kunihiro Funasaka

• Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan

author

Masaaki Kitano

• Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan

author

Akihiko Nakama

• Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan

author

Taro Yoshikura

• Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan

author

Yoshimitsu Oda

• Osaka Prefectural Institute of Public Health, Osaka, Japan

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