Fibrin D-dimer impairs the accumulation and anticoagulant properties of heparan sulphate and stimulates secretion of plasminogen activator inhibitor-1 by rabbit coronary endothelial cells.

• Authors: Natalia Yu Yevdokimova , Larisa D Veselovska , Genrietta K Gogolinska , Olexandra M Buchanevich , Galina V Kosyakova , Pavel G Gritsenko , Eduard V Lugovskoj , Sergiy V Komisarenko
• Languages of publication: EN

Abstracts

EN

Fibrin split product D-dimer (DD) is most probably involved in the development of vascular disorders. At 1.5 μM concentration DD inhibited the incorporation of D-[1-3H]glucosamine hydrochloride and [2-14C]acetate · Na into pericellular heparan sulphate (HS) of rabbit coronary endothelial cells without affecting other groups of glycosaminoglycans (GAGs). At the same time, DD reduced HS ability to bind antithrombin (AT) and suppressed NO production. The effect of DD on pericellular GAGs was similar to that of Nw-methyl-L-arginine, the competitive inhibitor of endothelial NO synthase (eNOS). L-Ascorbic acid, eNOS activator, increased the level of endogenous NO in the DD-treated cells, and restored HS accumulation and antithrombin binding. It is suggested that DD influence on endothelial HS may be mediated by NO production. Another effect of DD, namely, stimulation of plasminogen activator inhibitor-1 (PAI-1) secretion did not depend on the NO level. The decreased HS content, reduced anticoagulant properties of HS, and increased PAI-1 secretion disorganized the endothelial matrix, and promoted fibrin formation and vascular damage. This points to DD as an important factor in the development of vascular disorders.

Keywords

EN

fibrin D-dimer; plasminogen activator inhibitor-1; endothelial cells; heparan sulphate

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 1
• Pages: 279-289

Dates

published

2003

received

2002-11-27

revised

2003-01-30

accepted

2003-02-24

Contributors

author

Natalia Yu Yevdokimova

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Larisa D Veselovska

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Genrietta K Gogolinska

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Olexandra M Buchanevich

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Galina V Kosyakova

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Pavel G Gritsenko

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Eduard V Lugovskoj

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

author

Sergiy V Komisarenko

• Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

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