Cloning and characterization of a Schistosoma mansoni 1H and 30S clones as two tegumental vaccine candidate antigens

• Authors: Shabaan M Amr , Mohamed M Magdy , Abdallah S Mohga , Ibrahim M Hayat , Karim M Amr
• Languages of publication: EN

Abstracts

EN

Two Schistosoma mansoni cDNA clones 30S and 1H were identified by immunoscreening of sporocyst λgt11 library and by random sequencing of clones from λZap libraries, respectively. Clone 30S was one of 30 clones identified by an antibody raised against tegument of 3-h schistosomules. The clone was found to encode an 81 amino-acid protein fragment. It was expressed in Escherichia coli as a fusion protein of calculated molecular mass of about 35 kDa with C-terminus of Schistosoma japonicum glutathione-S-transferase (Sj26; about 26 kDa). The recombinant fusion protein was specifically recognized by serum of rabbits immunized with irradiated cercariae. Clone 1H is one of 76 expressed sequence tags derived from an adult worm library. It encodes the complete sequence of a tegumental membrane protein, Sm13. The 104 amino-acid open reading frame encodes a protein with a calculated molecular mass of about 11.9 kDa. Clone 1H was expressed in E. coli as an insoluble fusion protein with Sj26 of about 40 kDa. In Western blots, the fusion protein was recognized by serum from rabbits vaccinated with irradiated cercariae but not by preimmune rabbit sera. The cloning, characterization and expression of those proteins are therefore potentially usefull for vaccine development.

Keywords

EN

anti-GST; Tegumental antigen; vaccine candidate antigen; Sm13

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 1
• Pages: 269-278

Dates

published

2003

received

2002-04-10

revised

2002-10-29

accepted

2003-02-18

Contributors

author

Shabaan M Amr

• Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

author

Mohamed M Magdy

• Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

author

Abdallah S Mohga

• Department of Chemistry, Faculty of Science, Helwan University, Cairo, Egypt

author

Ibrahim M Hayat

• Department of Chemistry, Faculty of Science, Helwan University, Cairo, Egypt

author

Karim M Amr

• Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

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