Defective Brca2 influences topoisomerase I activity in mammalian cells*.

• Authors: Iwonna Rahden-Staroń , Maria Szumiło , Emilia Grosicka , Maria Kraakman - van der Zwet , Małgorzata Z Zdzienicka
• Languages of publication: EN

Abstracts

EN

The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.; 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the observed changes in the topoisomerase I activity in V-C8 are due to the defective function of the Brca2 gene.

Keywords

EN

camptothecin; V-C8 mutant; topoisomerase I; Brca2 gene

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 1
• Pages: 139-144

Dates

published

2003

received

2003-01-13

revised

2003-02-17

accepted

2003-03-05

Contributors

author

Iwonna Rahden-Staroń

• Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Maria Szumiło

• Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Emilia Grosicka

• Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Maria Kraakman - van der Zwet

• Department of Radiation Genetics and Chemical Mutagenesis-MGC, Leiden University Medical Center, Leiden, The Netherlands

author

Małgorzata Z Zdzienicka

• Department of Molecular Cell Genetics, The L. Rydygier University of Medical Sciences, Bydgoszcz, Poland

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