RFLP analysis of 1-aminocyclopropane-1-carboxylate synthase ACC2 and ACC4 genes from Polish cultivars of tomato.

• Authors: Małgorzata Jakubowicz , Justyna Jółkowska , Grażyna Kwapiszewska , Anna Kręcisz , Piotr Czerski
• Languages of publication: EN

Abstracts

EN

An important trait of tomato is the rate of fruit ripening, strongly dependent on ethylene production. The ripening-related ethylene synthesis in tomato is controlled mainly by 1-aminocyclopropane-1-carboxylate synthase LE-ACS2 and LE-ACS4 isoenzymes (Rottmann et al., 1991, J. Mol. Biol. 222: 937; Lincoln et al., 1993, J. Biol. Chem. 268: 19422; Barry et al., 2000, Plant Physiol. 123: 979). In spite of numerous reports on the LE-ACS2 and LE-ACS4 gene expression, only ones considered the genomic organisation each of these genes (Rottmann et al., 1991; Lincoln et al., 1993) reported one copy of each of these genes in tomato cv VF36. In this article we suggest that the genomic organisation of LE-ACS2 and LE-ACSS4 genes may depend on tomato cultivars and may differ from that described by the above authors. The results of Southern analyses of genomic DNAs from 17-day old seedlings (cultivars Jaga, Halicz, Betalux, New Yorker) imply that the genomic organisation of LE-ACS2 and LE-ACS4 genes in Polish cultivars differs from that reported for cv VF36.

Keywords

EN

1-aminocyclopropane-1-carboxylate synthase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 4
• Pages: 1037-1042

Dates

published

2002

received

2002-03-15

revised

2002-06-18

accepted

2002-11-12

Contributors

author

Małgorzata Jakubowicz

• Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Międzychodzka 5, 60-371 Poznań, Poland

author

Justyna Jółkowska

• Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Międzychodzka 5, 60-371 Poznań, Poland

author

Grażyna Kwapiszewska

• Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Międzychodzka 5, 60-371 Poznań, Poland

author

Anna Kręcisz

• Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Międzychodzka 5, 60-371 Poznań, Poland

author

Piotr Czerski

• Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Międzychodzka 5, 60-371 Poznań, Poland

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