Carbohydrate moieties of N-cadherin from human melanoma cell lines.

• Authors: Dorota Ciołczyk-Wierzbicka , Dorota Gil , Dorota Hoja-Łukowicz , Anna Lityńska , Piotr Laidler
• Languages of publication: EN

Abstracts

EN

Expression of N-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential. Many studies suggested the role of N-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion. N-cadherin is a heavily glycosylated protein. We have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: WM35 from the primary tumor site and WM239, WM9, and A375 from different metastatic sites. N-cadherin was immunoprecipitated with anti-human N-cadherin polyclonal antibodies. Characterisation of its carbohydrate moieties was carried out by SDS/PAGE electrophoresis and blotting, followed by immunochemical identification of the N-cadherin polypeptides and analysis of their glycans using highly specific digoxigenin or biotin labelled lectins. The positive reaction of N-cadherin from the WM35 cell line with Galanthus nivalis agglutinin (GNA), Datura stramonium agglutinin (DSA) and Sambucus nigra agglutinin (SNA) indicated the presence of high-mannose type glycans and biantennary complex type oligosaccharides with α2-6 linked sialic acid. N-cadherin from WM239, WM9, and A375 cell lines gave a positive reaction with Phaseolus vulgaris leukoagglutinin (L-PHA) and lotus Tetragonolobus purpureas agglutinin (LTA). This indicated the presence of tri- or tetra-antennary complex type glycans with α-fucose. In addition, N-cadherin from WM9 (lymphomodus metastatic site) and A375 (solid tumor metastatic site) contained complex type chains with α2-3 sialic acid (positive reaction with Maackia amurensis agglutinin - MAA). The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells.

Keywords

EN

melanoma; N-cadherin; N-glycans

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 4
• Pages: 991-998

Dates

published

2002

received

2002-04-22

revised

2002-10-17

accepted

2002-11-19

Contributors

author

Dorota Ciołczyk-Wierzbicka

• Institute of Medical Biochemistry, Medical College, Jagiellonian University, Kraków, Poland

author

Dorota Gil

• Institute of Medical Biochemistry, Medical College, Jagiellonian University, Kraków, Poland

author

Dorota Hoja-Łukowicz

• Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland

author

Anna Lityńska

• Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland

author

Piotr Laidler

• Institute of Medical Biochemistry, Medical College, Jagiellonian University, Kraków, Poland

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