Up-regulation of spermidine/spermine N1-acetyltransferase (SSAT) expression is a part of proliferative but not anabolic response of mouse kidney.

• Authors: Magdalena Dudkowska , Agnieszka Stachurska , Barbara Grzelakowska-Sztabert , Małgorzata Manteuffel-Cymborowska
• Languages of publication: EN

Abstracts

EN

A differential expression pattern of spermidine/spermine N1-acetyltransferase (SSAT), the enzyme critical to proper homeostasis of cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity and SSAT mRNA are significantly induced by antifolate CB 3717 and folate that evoke a drug-injury-dependent hyperplasia. In contrast, SSAT activity is down-regulated in the testosterone-induced hypertrophic kidney, while SSAT mRNA is positively controlled by this androgen. Catecholamine depletion evoked by reserpine drastically decreases the folate-induced activity of S-adenosylmethionine decarboxylase (AdoMetDC), which limits polyamine biosynthesis, but has no effect on SSAT activity augmented by CB 3717. Our results document that the increased SSAT expression solely accompanies the proliferative response of mouse kidney, and suggest the importance of post-transcriptional regulation to the control of SSAT activity in both hyperplastic and hypertrophic experimental models.

Keywords

EN

folic acid; antifolate CB 3717; polyamine metabolism; testosterone; polyamine homeostasis; reserpine; SSAT

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 4
• Pages: 969-977

Dates

published

2002

received

2002-10-10

revised

2002-11-12

accepted

2002-12-04

Contributors

author

Magdalena Dudkowska

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Agnieszka Stachurska

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Barbara Grzelakowska-Sztabert

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Małgorzata Manteuffel-Cymborowska

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

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