The structure of the oligosaccharides of α3β1 integrin from human ureter epithelium (HCV29) cell line.

• Authors: Anna Lityńska , Ewa Pocheć , Dorota Hoja-Łukowicz , Elżbieta Kremser , Piotr Laidler , Angela Amoresano , Chiara Monti
• Languages of publication: EN

Abstracts

EN

There is a growing line of evidence that glycosylation of α and β subunits is important for the function of integrins. Integrin α3β1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin α3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.

Keywords

EN

glycosylation; α3β1 integrin; cell line; MALDI MS

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 2
• Pages: 491-500

Dates

published

2002

received

2001-11-20

revised

2002-02-20

accepted

2002-04-10

Contributors

author

Anna Lityńska

• Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Ewa Pocheć

• Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Dorota Hoja-Łukowicz

• Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Elżbieta Kremser

• Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Piotr Laidler

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Angela Amoresano

• Centro Internazionale di Servizi di Spettometria di Massa, Napoli, Italy

author

Chiara Monti

• Centro Internazionale di Servizi di Spettometria di Massa, Napoli, Italy

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