Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.

• Authors: Zuzanna Dobrzańska , Joanna Więckiewicz , Jacek Bigda
• Languages of publication: EN

Abstracts

EN

In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.

Keywords

EN

PCR primers; p75 TNF-R; TNF; hamster

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 2
• Pages: 427-431

Dates

published

2002

received

2002-01-02

revised

2002-04-2

accepted

2002-05-23

Contributors

author

Zuzanna Dobrzańska

• Department of Histology and Immunology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

author

Joanna Więckiewicz

• Department of Histology and Immunology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

author

Jacek Bigda

• Department of Histology and Immunology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

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