Influence of organic solvents on papain kinetics

• Authors: Mariusz Szabelski , Krystyna Stachowiak , Wiesław Wiczk
• Languages of publication: EN

Abstracts

EN

Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMF and MeOH concentration.

Keywords

EN

organic solvent; fluorogenic substrate; enzyme activity; papain

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1197-1201

Dates

published

2001

received

2001-09-26

accepted

2001-11-27

Contributors

author

Mariusz Szabelski

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Krystyna Stachowiak

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Wiesław Wiczk

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

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