Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry.

• Authors: Piotr Stefanowicz , Janusz Boratyński , Urszula Kańska , Inga Petry , Zbigniew Szewczuk
• Languages of publication: EN

Abstracts

EN

Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation prod- ucts of proteins and peptides. We discuss influence of reaction conditions on the rate of glycation of lysozyme. We analyze for the first time collision induced dissociation spectra of the obtained peptide conjugates.

Keywords

EN

modified peptides; lysozyme; ESI-MS; glycation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1137-1141

Dates

published

2001

received

2001-09-19

accepted

2001-11-29

Contributors

author

Piotr Stefanowicz

• Faculty of Chemistry, University of Wrocław, Wrocław, Poland

author

Janusz Boratyński

• Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Urszula Kańska

• Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Inga Petry

• Faculty of Chemistry, University of Wrocław, Wrocław, Poland

author

Zbigniew Szewczuk

• Faculty of Chemistry, University of Wrocław, Wrocław, Poland

References

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