Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry—apowerful tool for peptide and protein identification.

• Authors: Margareta Ramström , Magnus Palmblad , Ardeshir Amirkhani , Youri O Tsybin , Karin E Markides , Per Håkansson , Jonas Bergquist
• Languages of publication: EN

Abstracts

EN

In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92% the protein.

Keywords

EN

liquid-chromatography; peptides; Fourier transform ion cyclotron resonance mass spectrometry; proteins

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1101-1104

Dates

published

2001

received

2001-09-19

accepted

2001-11-09

Contributors

author

Margareta Ramström

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden

author

Magnus Palmblad

• Division of Ion Physics, The Ångström Laboratory, Uppsala University, Uppsala, Sweden

author

Ardeshir Amirkhani

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden

author

Youri O Tsybin

• Division of Ion Physics, The Ångström Laboratory, Uppsala University, Uppsala, Sweden

author

Karin E Markides

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden

author

Per Håkansson

• Division of Ion Physics, The Ångström Laboratory, Uppsala University, Uppsala, Sweden

author

Jonas Bergquist

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden

References

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• 3. Nilsson, S., Wetterhall, M., Bergquist, J., Nyholm, L. & Markides, K. (2001) Rapid Commun. Mass Spectrom. 15, 1997-2000.
• 4. Palmblad, M., Wetterhall, M., Markides, K., Håkansson, P. & Bergquist, J. (2000) Rapid Commun. Mass Spectrom. 14, 1029-1034.