The effects of galactosamine on UTP levels in the livers of young, adult and old rats.

• Authors: Zbigniew Kmieć , Ryszard T Smoleński , Marek Zych , Andrzej Myśliwski
• Languages of publication: EN

Abstracts

EN

Galactosamine (GalN), a well-known hepatotoxin that depletes the cellular pool of uracil nucleotides, was previously shown to have greater impact on the inhibition of protein synthesis in hepatocytes of old rats as compared with young animals (Kmieć 1994, Ann. N.Y. Ac. Sci. 717, 216-225). In the present study we compared the effects of GalN on the nucleotide content (measured by ion-exchange HPLC) in the livers of young (4 months), adult (12 months), and old (24-26 months old) rats two hours after its intraperitoneal administration. UTP content of the livers of old control rats was significantly lower (by 28%) than that of young animals. GalN administration decreased the UTP content in the livers of young, adult and old rats by, respectively, 55%, 65% and 89%, and increased the content of UDP-sugars by 189%, 175% and 305%. The hepatic content of ATP, ADP, AMP, NAD, GTP except CTP did not differ significantly among the age groups of rats studied, and was not changed by GalN treatment. The content of CTP was significantly higher in old rats (P < 0.03) upon GalN treatment. The lower hepatic content of UTP may partially explain the increased sensitivity of hepatocytes and livers of old rats to the action of galactosamine, and possibly to other hepatotoxic compounds that decrease transcription in the liver.

Keywords

EN

liver; UTP; rat aging; galactosamine; nucleotides

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 2
• Pages: 349-353

Dates

published

2000

received

2000-04-26

Contributors

author

Zbigniew Kmieć

• Department of Histology and Immunology, University Medical School of Gdańsk, Gdańsk, Poland

author

Ryszard T Smoleński

• Department of Biochemistry, University Medical School of Gdańsk, Gdańsk, Poland

author

Marek Zych

• Department of Biochemistry, University Medical School of Gdańsk, Gdańsk, Poland

author

Andrzej Myśliwski

• Department of Histology and Immunology, University Medical School of Gdańsk, Gdańsk, Poland

References

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