Cytostatic and cytotoxic effects of (E)-2'-deoxy-2'-(fluoromethylene)-cytidine on a solid tumor and a leukemia cell line.

• Authors: Paweł Grieb , Mirosława Koronkiewicz , Janusz S Skierski
• Languages of publication: EN

Abstracts

EN

(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a human colorectal carcinoma line COLO-205. Despite dCK activity being by far the highest in cells of lymphoid origin, the effects of FMdC were detectable at the lowest drug concentration only in a solid tumor cell line, and at higher concentrations they were qualitatively similar in the two tumor lines (increased cell protein content, cell cycle block and apoptosis). Apparently, low dCK activity in solid tumor cells sufficiently activates FMdC to yield cytotoxic effects, while high dCK activity in leukemia cells does not increase its cytotoxicity.

Keywords

EN

cytotoxicity; flow cytometry; fluoromethylenedeoxycytidine; apoptosis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 1
• Pages: 165-171

Dates

published

2000

received

1999-10-25

Contributors

author

Paweł Grieb

• Laboratory of Experimental Pharmacology, Medical Research Center, Polish Academy of Sciences, Warszawa, Poland

author

Mirosława Koronkiewicz

• Flow Cytometry Laboratory, Drug Institute, Warszawa, Poland

author

Janusz S Skierski

• Flow Cytometry Laboratory, Drug Institute, Warszawa, Poland

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