The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome P450 reductase and xanthine oxidase

• Authors: Jolanta Pawłowska , Jolanta Tarasiuk , Edward Borowski , Małgorzata Wąsowska , Irena Oszczapowicz , C Roland Wolf
• Languages of publication: EN

Abstracts

EN

Some sterically hindered N-substituted derivatives of daunorubicin are known to be poor substrates for NADH dehydrogenase, NADPH cytochrome P450 reductase and xanthine oxidase. In consequence, poor oxygen radical generation by these compounds is observed. In this study we examined a new family of sugar-N-substituted derivatives of daunorubicin bearing a bulky substituent introduced on the nitrogen atom through the amidine spacer. These compounds were found to be very active in radical formation catalyzed by all three studied enzymes. Thus, the introduction of a heterocyclic ring, even if it is bulky but flexible, on the nitrogen atom of daunosamine moiety through the one-atom spacer (amidine group), does not induce the steric hindrance effect on the interaction of daunorubicin derivatives with these flavoprotein enzymes.

Keywords

EN

xanthine oxidase; NADH dehydrogenase; daunorubicin; formamidine derivatives; free radical formation; NADPH cytochrome P540 reductase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 1
• Pages: 141-147

Dates

published

2000

received

1999-10-25

Contributors

author

Jolanta Pawłowska

• Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, G. Narutowicza 11/12, 80-952 Gdańsk, Poland

author

Jolanta Tarasiuk

• Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, G. Narutowicza 11/12, 80-952 Gdańsk, Poland

author

Edward Borowski

• Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, G. Narutowicza 11/12, 80-952 Gdańsk, Poland

author

Małgorzata Wąsowska

• Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516 WarszawaPoland

author

Irena Oszczapowicz

• Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516 WarszawaPoland

author

C Roland Wolf

• Molecular Pharmacology Unit, Biomedical Research Centre, Ninewells Hospital and Medical School, Dundee, U.K.

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