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2017 | 89 | 48-63
Article title

Isolation and characterization of some dominant yeast strains for production of bioethanol from Arabica coffee (Coffea arabica L.) wet processing wastes

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The current study was initiated to isolate and characterize yeasts from wet Arabica coffee processing wastes for bioethanol production. Yeast isolates were retrieved from wet Arabica coffee processing effluent1, effluent2, effluent3, pulp1 and pulp2. The yeast isolates were screened and characterized for ethanol production following standard methods. All the isolates were first tested for carbohydrate fermentation in appropriate medium. Selected ethanol producing isolates were tested for various parameters. Yeast isolates from pulp (ACP12) and effluent (ACE12) showed significantly high counts at 20% glucose concentration with the highest population number of 2.16 ±1.00×108 and 1.21±1.00×108 CFU/ml, respectively. Isolate ACP12 showed higher population number (9.7±1.00×107 CFU/ml) than the standard culture (Saccharomyces cerevisiae) with colonies count of 8.7±1.00×107 CFU/ml at 30 °C. Moreover, yeast isolate (ACP12) showed higher colonies count (9.8 ±1.00×107 CFU/ml) compared to the standard strain (8.7±1.00 ×107 CFU/ml) at pH 5.0. Based on morphological, physiological and biochemical characteristics, the two isolates (ACE12 and ACP12) were tentatively identified as genus Saccharomyces. Total sugar concentration on (90%) was obtained from pulp1. Isolate ACP12 showed the maximum ethanol production (6.2 g/l) from pulp1 compared to the standard isolate (5.49 g/l) and the other test yeasts. From this study, it can be concluded that isolate ACP12 has an inherent potential of ethanol production from coffee pulps compared to the rest yeast isolates and needs further supplementary activities to qualify it for industrial application.
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  • Department of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia
  • Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia
  • [1] Ali, M.N and Khan, M.M. (2014). Screening, identification and characterization of alcohol tolerant potential bioethanol producing yeasts. Current Research in Microbiology and Biotechnology. 2: 316-324.
  • [2] Ayele Kefale. (2011). Bioethanol Production and Optimization test from Agricultural Waste: The case of wet coffee processing waste (pulp). M.Sc. Thesis. AAU.
  • [3] Barnett, J.A, Payne, R.W, Yarrow, D. (2000). Yeasts: Characteristics and identification. 3rd Edition, Cambridge University Press, UK.
  • [4] Bekatorou, A., Psarianos, C. and Koutinas, A.A. (2006). Production of food grade yeasts. Food Technol. Biotechnol. 44: 407- 415.
  • [5] Berndes , G.M. Hoogwijk and R.Van den Broek. The contribution of biomass in the future global energy supply: a review of 17 studies. Biomass and Bioenergy. 25: 1-28, 2003.
  • [6] Birhanu Abegaz Gashe, Meaza Girma and Abrham Bisrat .(1982). The role of microorganisms in fermentation and their effect on the nitrogen content of teff. SINET: Ethiop. J. Sci. 5: 69-76.
  • [7] Boekhout, T. and Kurtzman, C.P. (1996). Principles and methods used in Yeast classification, and over view of currently accepted yeast genera. In: Non conventional yeasts in bio technology. A Hand book; Springer Velag, Berlin, Heidelberg (Wolf). 1- 99.
  • [8] Casey, G.P. and Ingledew, W.M. (1986). Ethanol tolerance in yeasts. CRC .Crit. Rev. Microbiol. 13: 219-280.
  • [9] Davis, L., Jeon, Y. and Svenson, C. (2005). Evaluation of wheat stillage for ethanol production by recombinant Zymomonas mobilis. Biomass Bioenerg. 29: 49-59.
  • [10] Deepa, G.B., Chanakya, H.N., de Alwis, A.A.P., Manjunath, G.R., Devi, V. (2002). Overcoming Pollution of Lakes and Water Bodies Due to Coffee Pulping Activities with Appropriate Technology Solutions. Proceedings Symposium on Conservation, Restoration and Management of Aquatic Ecosystems. Canada: Centre for Ecological Sciences, Indian Institute of Science (IIS) and the Karnataka Environment Research Foundation (KERF), Bangalore and Common wealth of Learning.
  • [11] Demirbas, F., Bozbas, K. and Balat, M. (2004). Carbon dioxide emission trends and environmental problems in Turkey. Energy Explor. Exploit. 22: 355-365.
  • [12] Demirbas, M.F. (2006). Global renewable Energy Resources. Energy Sources 28: 779 792.
  • [13] Franca, A., Gouvea, B., Torres, C., Oliveira, L. and Oliveira, E. (2008). Feasibility of ethanol production from coffee husks. J. Biotechnol. 136: 269-275.
  • [14] Kumar, A. 2012. Next generation bio-fuels for greenhouse gas mitigation and role of biotechnology. Emerging trends in biotechnology and pharmaceutical research, Mangalayatan University, 38.
  • [15] Linden, T., J. Peetre and B.H. Hagerdal. (1992). Isolation and characterization of acetic acid tolerant galactose fermenting strains of Saccharomyces cerevisiae from a spent liquor fermentation plant. Appl. Environ. Microbiol. 58: 1661-1669.
  • [16] Lodder, J. (1971). The Yeasts. A taxonomic Study. North Holland Pub. Co. Amsterdam.
  • [17] Osho, A. (2005). Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice. Afr. J. Biotechnol. 4: 660-662.
  • [18] Samuel Sahle and Birhanu Abegaz Gashe, (1991). Microbiology of tella fermentation SINET: Ethiop. J. Sci. 14: 81- 92.
  • [19] Subashini, D., J. Ejilane, A. Radha, M.A. Jayasri and K. (2011). Suthindhiran School of Biosciences and Technology, VIT University, Tamil Nadu, India
  • [20] Sun, Y. and Cheng, J. (2002). Hydrolysis of lignocellulosic materials for ethanol production: a review. Biores. Technol. 83: 1-11.
  • [21] Sree, N., Sridhar, M., Suresh, K., Bharat, I. and Rao, L. (2000). High alcohol production by repeated batch fermentation using immobilized osmotolerant S. cerevisiae. J. Indust. Microbiol. Biotechnol. 24: 222-226.
  • [22] Teramoto, Y. Sato, R. and Ueda, S. (2005). Characteristics of fermentation yeast isolated from traditional Ethiopian honey wine, ogol. Afr .J. Biotechnol. 4: 160-163.
  • [23] Turhan, I., Demirci, A.; Karhan, M. (2010). Ethanol production from carob extract by Saccharomyces Cerevisiae. Bioresource Technol. 101: 5290-5296.
  • [24] Walker, L.P, Hii, H. Wilson, D.B. (2006). Enzymatic hydrolysis of cellulose: An Overview. Biores. Technol. 36: 3-14.
  • [25] Warren, P. and Shadomy, L. (1991). Yeast fermentation broth base with carbohydrate and Durham Tube. In: Manual of Clinical Microbiology.5th ed. Washington D.C.
  • [26] Wildenborg, T. and Lokhorst, A.(2005). Introduction on CO2 Geological storage-classification of storage options. Oil Gas Sci. Technol. Rev. 60: 513-515.
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