Isolation and characterization of some dominant yeast strains for production of bioethanol from Arabica coffee (Coffea arabica L.) wet processing wastes
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The current study was initiated to isolate and characterize yeasts from wet Arabica coffee processing wastes for bioethanol production. Yeast isolates were retrieved from wet Arabica coffee processing effluent1, effluent2, effluent3, pulp1 and pulp2. The yeast isolates were screened and characterized for ethanol production following standard methods. All the isolates were first tested for carbohydrate fermentation in appropriate medium. Selected ethanol producing isolates were tested for various parameters. Yeast isolates from pulp (ACP12) and effluent (ACE12) showed significantly high counts at 20% glucose concentration with the highest population number of 2.16 ±1.00×108 and 1.21±1.00×108 CFU/ml, respectively. Isolate ACP12 showed higher population number (9.7±1.00×107 CFU/ml) than the standard culture (Saccharomyces cerevisiae) with colonies count of 8.7±1.00×107 CFU/ml at 30 °C. Moreover, yeast isolate (ACP12) showed higher colonies count (9.8 ±1.00×107 CFU/ml) compared to the standard strain (8.7±1.00 ×107 CFU/ml) at pH 5.0. Based on morphological, physiological and biochemical characteristics, the two isolates (ACE12 and ACP12) were tentatively identified as genus Saccharomyces. Total sugar concentration on (90%) was obtained from pulp1. Isolate ACP12 showed the maximum ethanol production (6.2 g/l) from pulp1 compared to the standard isolate (5.49 g/l) and the other test yeasts. From this study, it can be concluded that isolate ACP12 has an inherent potential of ethanol production from coffee pulps compared to the rest yeast isolates and needs further supplementary activities to qualify it for industrial application.
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