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2021 | 75 | 18-23
Article title

Centrally acting cholecystokinin induces depressor circulatory effects in haemorrhage-shocked rats

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PL
Ośrodkowo działająca cholecystokinina wywołuje działanie depresyjne na układ krążenia u szczurów we wstrząsie krwotocznym
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Abstracts
EN
INTRODUCTION: Cholecystokinin (CCK) is a peptide gastrointestinal hormone involved in the stimulation of lipid and protein digestion as well as a neurotransmitter/neuromodulator in the central nervous system. After intravenous administration, it induces a resuscitating effect in rats subjected to haemorrhagic shock. Since CCK is able to directly and indirectly affect the cardiovascular centre function, the aim of the study was to examine the action of the sulphated octapeptide form of CCK (CCK-8) given intracerebroventricularly (icv) in the sympathoinhibitory phase of haemorrhagic shock. MATERIAL AND METHODS: Studies were carried out in male Wistar rats anaesthetized with ketamine/xylazine (100 mg/kg + 10 mg/kg, intramuscularly) and subjected to irreversible haemorrhagic shock (0% survival at 2 h) with a mean arterial pressure (MAP) of 20–25 mmHg. At 5th min of critical hypotension, the rats were injected icv with CCK-8 (5, 15 nmol) or saline (5 μl). RESULTS: Haemorrhage led to a decrease in pulse pressure (PP), heart rate (HR) as well as increases in renal (RVR) and mesenteric vascular resistance (MVR). In the control group injected with saline, there were no significant increases in the measured cardiovascular parameters, and the survival time was 32.5 ± 5.1 min. CCK-8 induced dose-dependent decreases in MAP, PP and HR accompanied by increases in RVR and MVR, and also shortened the survival time in comparison to the control animals. CONCLUSIONS: Centrally acting CCK-8 induces depressive circulatory effects in haemorrhage-shocked rats.
PL
WSTĘP: Cholecystokinina (CCK) należy do hormonów peptydowych układu pokarmowego regulujących trawienie lipidów i białek, a ponadto jest ośrodkowym neurotransmiterem/neuromodulatorem. Po podaniu dożylnym wywołuje efekt resuscytacyjny u szczurów we wstrząsie krwotocznym. Ze względu na fakt, iż CCK może wpływać bezpośrednio i pośrednio na czynność ośrodka sercowo-naczyniowego, celem pracy było zbadanie działania pochodnej siarczanowej oktapeptydu CCK (CCK-8) podawanej do komory bocznej mózgu (intracerebroventricularly – icv) w fazie hamowania czynności układu współczulnego we wstrząsie krwotocznym. MATERIAŁ I METODY: Badania przeprowadzono u samców szczurów szczepu Wistar w znieczuleniu ogólnym (ketamina [100 mg/kg]/ksylazyna [10 mg/kg]), u których wywołano nieodwracalny wstrząs krwotoczny (0% przeżycia 2 h) ze średnim ciśnieniem tętniczym (mean arterial pressure – MAP) 20–25 mmHg. W 5 min krytycznej hipotensji szczurom podawano icv CCK-8 (5, 15 nmol) lub 0,9% roztwór NaCl (5 μl). WYNIKI: Krwotok prowadził do obniżenia ciśnienia tętna (pulse pressure – PP), częstości rytmu serca (heart rate – HR) oraz wzrostu nerkowego (renal vascular resistance – RVR) i krezkowego oporu naczyniowego (mesenteric vascular resistance – MVR). W grupie kontrolnej nie stwierdzono wzrostu badanych parametrów układu krążenia, a średni czas przeżycia wynosił 32,5 ± 5,1 min. CCK-8 wywoływała zależne od dawki spadki MAP, PP i HR ze wzrostem RVR i MVR, a także skracała czas przeżycia w porównaniu ze zwierzętami kontrolnymi. WNIOSKI: Ośrodkowo działająca CCK-8 wywołuje działanie depresyjne na układ krążenia u szczurów we wstrząsie krwotocznym.
Discipline
Year
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75
Pages
18-23
Physical description
Contributors
  • Katedra i Zakład Fizjologii, Wydział Nauk Medycznych w Zabrzu, Śląski Uniwersytet Medyczny w Katowicach
  • Wydział Lekarski, Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu
author
  • Katedra i Zakład Fizjologii, Wydział Nauk Medycznych w Zabrzu, Śląski Uniwersytet Medyczny w Katowicach
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Document Type
article
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YADDA identifier
bwmeta1.element.psjd-febba1d1-f3df-4700-bf99-5b09a72644c5
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