A preliminary comparative study of root – associated microbial communities in plantain (Musa paradisiaca L.) grown in natural and herbicide polluted soils in Toru Orua, Bayelsa State

• Authors: Philips Ibuomotimi Asaigbe , Alice Ebimoboere Manda , Funlayefa Atoukudu
• Languages of publication: EN

Abstracts

EN

This preliminary comparative study was conducted to assess the impact of herbicides on the microbial communities of plantain (Musa paradisiaca L.). Rhizospheric samples were collected from natural and herbicide polluted soils within the campus of the University of Africa, Toru-Orua, Bayelsa State, Nigeria. Samples were serial diluted where 10-6 and 10-7 were adopted. Microbial populations in both herbicide polluted soil (HP) and Normal Soil (NS) were carefully compared on PDA, MAC and NA media and morphological characteristics differences were carefully analysed. The results revealed clear differences between HP soil and NS. The HP soil sample had a significantly higher overall microbial load and species richness compared to the NS sample. Total culturable bacteria in HP soil made up 43.75% of the isolates while NS soil had 25%. In HP soil, fungi constituted 25% of the isolates while only 6.25% fungi were found in NS soil. key organisms isolated include Cryptococcus spp. and Fusarium spp. (pathogenic) were more in occurrence than other commensal fungi such as Rhizopus spp., Basidiomycetes, Aspergillus spp. where Basidiomycetes which are known decomposers. Bacterial isolates are Micrococcus luteus (mostly abundant) Enterobacter spp, Enterobacter aerogenes, Bacillus spp., Proteus spp. and Pseudomonas spp. While studies have reported a decline in overall microbial presence and diversity after herbicide use due to general toxicity, the increased abundance and richness in the HP soil reflects an ecological trend of selective adaptation as previously reported by a few authors. The study concludes that the use of herbicides as effective weed control, compromises the microbial balance of the plantain's rhizosphere. Thus, herbicide application does not directly destroy the plant microbiome but creates an imbalance that lead to the elimination of organisms which are not tolerant and the subsequent selective promotion of herbicide degrading microorganisms.

Keywords

EN

Food Security; Herbicide pollution; Plant microbiome; Plantain; Rhizosphere

Discipline

• BIOLOGY: BIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2026
• Volume: 64
• Pages: 275-289

Contributors

author

Philips Ibuomotimi Asaigbe

• Department of Biological Sciences, University of Africa, Toru-Orua, Bayelsa, Nigeria

author

Alice Ebimoboere Manda

• Department of Biological Sciences, University of Africa, Toru-Orua, Bayelsa, Nigeria

author

Funlayefa Atoukudu

• Department of Biological Sciences, University of Africa, Toru-Orua, Bayelsa, Nigeria

References

• [1] Adegboye, O. C. The role of beneficial microbes in sustainable plantain production in Nigeria. Nigerian Journal of Microbiology, 32(1), (2018) 45-56
• [2] Ahemad, M. & Kibret, M. Mechanisms and Applications of Plant Growth Promoting Rhizobacteria: Current Perspective. Journal of King Saud University - Science, 26, (2014) 1-20
• [3] Birt, H.W.G., Pattison, A.B., Skarshewski, A., Daniells, J., Raghavendra, A. and Dennis G. P. The core bacterial microbiome of banana (Musa spp.). Environmental Microbiome 17, (2022 a) 46
• [4] Birt, H. W. G., Tharp C. L., Custer, G. F., Dini-Andreote F. Root phenotypes as modulators of microbial microhabitats. Frontiers in Plant Science 13, (2022 b) 1-9
• [5] Cycoń M, Żmijowska A, Wójcik M, Piotrowska-Seget Z. Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils. Journal of Environmental Management 117, (2013) 7-16
• [6] Dinakarkumar, Y., Ramakrishnan, G., Gujjula, K.R., Vasu, V., Balamurugan, P. & Murali, G. Fungal bioremediation: An overview of the mechanisms, applications and future perspectives. Environmental Chemistry and Ecotoxicology, 6, (2024) 293-30
• [7] Iqbal, S., Begum, F., Nguchu, B.A., Claver, P. U. & Shaw, P. The invisible architects: microbial communities and their transformative role in soil health and global climate changes. Environmental Microbiome 20(36) (2025) 1-21
• [8] Kaushal, M., Kolombia, Y., Alakonya, A. E., Kuate, A. F., Alejandro Ortega Beltran, A. Amah, D. & Masso, C. Subterranean Microbiome Affiliations of Plantain (Musa spp.) Under Diverse Agroecologies of Western and Central Africa. Microbial Ecology 84 (2022) 580-593
• [9] Maikalu, R. B., Igere, B, E. & Odjadjare, E. E.O. Enterobacter species Distribution, emerging virulence and multiple antibiotic resistance dynamics in effluents: A countrified spread-hub and implications of abattior release. Total Environment Research Themes, 8 (2023) 1-9
• [10] Makut, M. D. & Bello, A. Assessment of the Biodegradation of Herbicides by Bacteria Isolated from the Soil. Asian Journal of Biotechnology and Bioresource Technology 4(1) (2018) 1-6
• [11] Matúš, P., Littera, P., Farkas, B., & Urík, M. Review on Performance of Aspergillus and Penicillium Species in Biodegradation of Organochlorine and Organophosphorus Pesticides. Microorganisms, 11(6) (2023) 1485
• [12] Moneke, A. N., Okpala, G. N., & Anyanwu, C. U. Biodegradation of glyphosate herbicide in vitro using bacterial isolates from four rice fields. African Journal of Biotechnology, 9(26) (2010) 4067-4074
• [13] Nwankwo, C. C., Arimaha C.O. and Ezeonuegbu, B. A. (2025). Comparative Analysis of the Physiochemical Characteristics and Bacterial Communities of Healthy and Infected Root Soil of Plantain Plant. Scientia Africana, 24 (2) (2025) 247-262
• [14] Ogunmwonyi I. N, Igbinosa O. E, Aiyegoro O. A and Odjadjare E. E. Microbial analysis of different top soil samples of selected site in Obafemi Awolowo University, Nigeria. Scientific Research and Essay 3(3) (2008) 120-124
• [15] Onwudiegwu, A. K., Okonofua, P. E., & Obe, O. J. Environmental and public health implications of pesticide residues: From soil contamination to policy interventions. Greener Journal of Medical Sciences, 15(1) (2025) 1-11
• [16] Pal, R., Chakrabarti, K., Chakraborty, A. & Chowdhury, A. Degradation and Effects of Pesticides on Soil Microbiological Parameters-A Review. International Journal of Agricultural Research, 1 (2006) 240-258
• [17] Papade, S. E,, Mohapatra, B. & Phale, P. S. Pseudomonas and Acinetobacter spp. capable of metabolizing aromatics displays multifarious plant growth promoting traits: Insights on strategizing consortium-based application to agro-ecosystems. Environmental Technology & Innovation, 36 (2024) 103786
• [18] Roy, B., Das, T. & Bhattacharyya, S. Overview on Old and New Biochemical Test for Bacterial Identification. Journal of Surgical Case Reports and Images 6(5) (2023) 1-11
• [19] Solanke, T., & Falade, O. A. The microbial diversity of plantain (Musa paradisiaca) root-associated soil in Southwestern Nigeria. Journal of Agricultural Science and Technology, 5(2) (2010) 112-119
• [20] Shah, B. A., Malhotra, H., Papade, S. E., Dhamale, T., Ingale, O. P., Kasarlawar, S. T., & Phale, P. S. Microbial degradation of contaminants of emerging concern: metabolic, genetic and omics insights for enhanced bioremediation. Frontiers in Bioengineering and Biotechnology, 12 (2024) 1470522
• [21] Teke E. C. & Akowei J. S. Assessment of microorganisms in the rhizosphere region of plantain in Bayelsa, Nigeria. World News of Natural Sciences, 50 (2023 a) 178-196
• [22] Teke, E. C. & Akowei, J. S.. Population, Morphological and Biochemical Characterization of Microorganism in Plantain Root across different Farmlands in Toru-Orua Metropolis, Bayelsa State, Nigeria. Journal of Applied Science and Environmental Management, 27(8) (2023 b) 1681-1685
• [23] Tyagi, S., Kumar, A., & Yadav, K. K. Effect of different herbicides on soil microbial population dynamics in Rabi maize (Zea mays L.). International Journal of Current Microbiology and Applied Sciences, 7(5) (2025) 1-9
• [24] Van der Heijden M. G, Bardgett, R. D, & van Straalen, N. M. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecology Letters 11(3) (2008) 296-310
• [25] Zhang, J., Cook, J., Nearing, J. T., Zhang, J., Raudonis, R. Bernard R. Glick, R. B., Morgan G.I. Langille, G. I. M. and Cheng, Z. Harnessing the plant microbiome to promote the growth of agricultural crops. Microbiological Research, 245 (2021) 126690

• Document Type: article